%0 Journal Article
%T 峨眉拟单性木兰嫩茎诱导及再生培养研究<br>Study on Shoot Induction and Tissue Culture Regeneration of Parakmeria omeiensi
%A 秦小波
%A 张国珍
%A 牛蓓
%A 徐莺
%J Botanical Research
%P 132-138
%@ 2168-5673
%D 2020
%I Hans Publishing
%R 10.12677/BR.2020.92016
%X <div style=\"text-align:justify;\">
	<span style=\"font-size:14px;line-height:1.5;\">为奠定人工繁育和保护研究的基础，对峨眉拟单性木兰嫩茎诱导培养、不定芽的分化及组培苗再生进行了研究，建立起培养周期短、再生效率高的峨眉拟单性木兰组织培养体系。利用多因子正交实验设计及分析，筛选出最佳诱导培养基是MS + 2.0 mg/L 6-BA + 1.5 mg/L KT + 0.02 mg/L IAA。通过比较植物生长调节剂，发现最适的分化培养基为MS + 0.5 mg/L 6-BA + 0.02 mg/L NAA。最后，生根培养基以MS培养基添加NAA 0.5 mg/L，IBA 2.5 mg/L能有效地使再生苗产生根系。<br />
Through the study of shoot induction culture, adventitious bud differentiation and plantlet rege-neration, a tissue culture system was established, which was short culture cycle and high regene-ration efficiency. The design and analysis of multivariate experiments showed, the best induction medium was MS + 2.0 mg/L 6-BA + 1.5 mg/L KT + 0.02 mg/L IAA. By comparing plant growth hor-mone, we found that the most suitable medium for differentiation was MS + 0.5 mg/L 6-BA + 0.02 mg/L NAA. Finally, MS medium with NAA 0.5 mg/L and IBA 2.5 mg/L can effectively produce roots of regenerated seedlings.</span> 
</div>
%K 拟单性木兰，嫩茎，再生，植物生长调节剂<br>Parakmeria omeiensis
%K Shoot
%K Regeneration
%K Plant Growth Hormone
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=34476