目的：通过高相液相色谱法建立一种快速测定洋金花中莨菪碱含量的方法。方法：采用SGE protecol C18 (5 μm, 4.6 × 250 mm)色谱柱，以乙腈-0.03 mol/L醋酸铵溶液(1:4, v/v)为流动相，其中醋酸铵溶液中含有三乙胺(0.02%)，四氢呋喃(0.3%)，加入乙酸调pH = 6，检测波长为216 nm，流速1.0 mL/min，柱温30℃，进样量20 μL。结果：洋金花中莨菪碱的浓度在62.5 μg/mL~1000 μg/mL范围内与峰面积呈现良好的线性关系，相关系数R = 0.998，该方法显著缩短莨菪碱的保留时间，并且精密度、稳定性、重复性的RSD均<2.0%，平均加样回收率为98.97%，RSD为0.39%。结论：本方法准确，重复性好，能够用于洋金花中莨菪碱含量的快速测定。
Objective: A method for the rapid determination of the content of hyoscyamine in Hindu datura was established by high-phase liquid chromatography. Method: SGE protecol C18 (5 μm, 4.6 × 250 mm) column was used with acetonitrile-0.03 mol/L ammonium acetate solution (1:4, v/v) as mobile phase. Ammonium acetate solution contained triethylamine (0.02%), tetrahydrofuran (0.3%), and adjusted to pH = 6 by adding acetic acid. The detection wavelength was 216 nm, the flow rate was 1.0 mL/min, the column temperature was 30℃, and the injection volume was 20 μL. Results: The concentration of hyoscyamine in the range of 62.5 μg/mL~1000 μg/mL showed a good linear relationship with the peak area, and the correlation coefficient was R = 0.998. The method signifi-cantly shortened the retention time of hyoscyamine, and the RSD of precision, stability and repea-tability were all <2.0%, the average recovery rate was 98.97%, and the RSD was 0.39%. Conclusion: The method is accurate and reproducible, and can be used for rapid determination of the content of hyoscyamine in Hindu datura.
