Nine microorganisms, comprising of four bacteria, Erwinia carotovora, Pseudomonas aeruginosa, Serratia marcescens, Klebsiella oxytoca and five fungi, Rhizopus stolonifera, Aspergillus niger, Aspergillus flavus, Fusarium oxysporum and Penicillium marneffei, isolated from rotten yam tubers of 2016 harvest year, were treated with ten plants extracts (Passiflora edulis, Daniella oliveri, Ceiba pentandra, Jatropha tanjorensis, Azadrichta indica, Carica papaya, Moringa oleifera, Mangifera indica, Terminalia catapa and Senna alata), singly and synergistically by incorporation of extract in media for inhibition test. Two plant extracts singly and completely inhibited the growth of three organisms: Terminalia catapa at 100% and 10-1 showed complete inhibition (a) of Erwinia carotovora. Passiflora edulis at undiluted (100%) concentration completely inhibited Rhizopus stolonifer and Penicillium marneffei, respectively. Synergistic plant extract recorded complete inhibition (a) of all the four bacteria isolates at 2 mL extract incorporation; ten (10) mL extract incorporation in media recorded complete inhibition (a) of three out of the five fungi isolates: Rhizopus stolonifer, Fusarium oxysporum and Penicillium marneffei, respectively; the other two fungi recorded high inhibition (b) of Aspergillus niger and Aspergillus flavus, respectively. Hot aqueous synergistic plants extract recorded poor inhibition of the isolates as compared to the cold. Soxhlet solvent extracted synergistic plants extract, however, recorded lower inhibition as compared to hot aqueous synergistic plants extract and cold aqueous synergistic plants extracts. Room temperature solvent extracted synergistic plants extracts recorded inhibition that was same as that obtained with cold aqueous synergistic plants extract. This research indicates that heat employed extractions recorded less inhibition activity.
References
[1]
Anjum, M.A., Ahmed, N., Babita, C.H. and Gupta, P. (2016) Plant Extracts in Post-Harvest Disease Management of Fruits and Vegetables—A Review. Journal of Food Processing and Technology, 7, 592.
https://doi.org/10.4172/2157-7110.1000592
[2]
FAO (2008) Food and Agricultural Organisation Corporate Statistical Database.
[3]
Adeniji, M.O. (1970) Fungi Associated with Storage Decay of Yam in Nigeria. Phytopathology, 60, 590-592. https://doi.org/10.1094/Phyto-60-590
[4]
Arene, O.B., Nwakiti, A.O. and Okafor, N. (1985) The Chemical Basis of the Pathology of Yam Tuber. In: Osuji, G., Ed., Advances in Yam Research, Biochemical Society of Nigeria, 362.
[5]
Okigbo, R.N. (2005) Biological Control of Post Harvest Fungal Rot of Yam (Dioscorea spp.) with Bacillus subtilis. Mycopathologia, 159, 307-314.
https://doi.org/10.1007/s11046-004-2454-8
[6]
Amusa, N.A., Adegbite, A.A., Muhammed, S. and Baiyewu, R.A. (2003) Yam Diseases and Its Management in Nigeria. African Journal of Biotechnology, 2, 497-502.
https://doi.org/10.5897/AJB2003.000-1099
[7]
Ijato, J.Y. (2011) Evaluation of Antifungal Effects of Extracts of Allium sativum and Nicotiana tobacum against Soft Rot of Yam (Dioscorea alata). Researcher, 3, 1-5.
http://www.sciencepub.net/researcher/research0302/01_3730research0302_1_5.pdf
Borris, R.P. (1996) Natural Products Research: Perspectives from a Major Pharmaceutical Company. Journal of Ethnopharmacology, 51, 29-38.
https://doi.org/10.1016/0378-8741(95)01347-4
[10]
Nascimento, G.G.F., Locatelli, J., Freitas, P.C. and Silva, G.L. (2000) Antibacterial Activity of Plant Extracts and Phytochemicals on Antibiotic-Resistant Bacteria. Brazilian Journal of Microbiology, 31, 247-256.
https://doi.org/10.1590/S1517-83822000000400003
[11]
Stockwell, C. (1988) Nature’s Pharmacy. Century Hutchinson Ltd., London.
[12]
Eisenberg, D.M., Kessler, R.C., Foster, C., Norlock, F.E., Calkins, D.R. and Delbanco, T.L. (1993) Unconventional Medicine in the United States: Prevalence, Costs and Patterns of Use. The New England Journal of Medicine, 328, 246-252.
https://doi.org/10.1056/NEJM199301283280406
[13]
Anesini, E. and Perez, C. (1993) Screening of Plants Used in Argentine Folk Medicine for Antimicrobial Activity. Journal of Ethnopharmacology, 39, 119-128.
https://doi.org/10.1016/0378-8741(93)90027-3
[14]
Martinez, M.J., Vasquez, S.M., Espinosa-Perez, C., Dias, M. and Herrera-Sanchez, M. (1994) Antimicrobial Properties of Argentatine A Isolated from Parthenium argentatum. Fitoterapia, 65, 371-372.
[15]
Martinez, M.J., Betancourt, J., Alonso-Gonzalez, N. and Jauregui, A. (1996) Screening of Some Cuban Medicinal Plants for Antimicrobial Activity. Journal of Ethnopharmacology, 52, 171-174. https://doi.org/10.1016/0378-8741(96)01405-5
[16]
Alonso-Paz, E., Cerdeiras, M.P., Fernandez, J., Ferreira, F., Moyna, P., Soubes, M., Vazquez, A., Veros, S. and Zunno, L. (1995) Screening of Uruguayan Medicinal Plants for Antimicrobial Activity. Journal of Ethnopharmacology, 45, 67-70.
https://doi.org/10.1016/0378-8741(94)01192-3
[17]
Devendra, B.N., Srinivas, N., Prasad Talluri, V.S.S. and Swarna, P.L. (2011) Antimicrobial Activity of Moringa oleifera Lam., Leaf Extract, against Selected Bacterial and Fungal Strains. International Journal of Pharma and Bio Sciences, 2, 13-18.
http://www.ijpbs.net
[18]
Abou-Karam, M. and Shier, W.T. (1990) A Simplified Plaque Reduction Assay for Antiviral Agents from Plants. Demonstration of Frequent Occurrence of Antiviral Activity in Higher Plants. Journal of Natural Products, 53, 340-344.
https://doi.org/10.1021/np50068a011
[19]
Afolayan, A.J. and Meyer, J.J.M. (1997) The Antimicrobial Activity of 3,5,7-Trihydroxyflavone Isolated from the Shoots of Helichrysum aureonitens. Journal of Ethnopharmacology, 57, 177-181.
https://doi.org/10.1016/S0378-8741(97)00065-2
[20]
Ahmad, A., Davies, J., Randall, S. and Skinner, G.R.B. (1996) Antiviral Properties of Extract of Opuntia streptacantha. Antiviral Research, 30, 75-85.
https://doi.org/10.1016/0166-3542(95)00839-X
[21]
Ahmed, A.A., Mahmoud, A.A., Williams, H.J., Scott, A.I., Reibenspies, J.H. and Mabry, T.J. (1993) New Sesquiterpene α-methylene Lactones from the Egyptian Plant Jasonia candicans. Journal of Natural Products, 56, 1276-1280.
https://doi.org/10.1021/np50098a011
[22]
Amoros, M., Simoes, C.M.O. and Girre, L. (1992) Synergistic Effect of Flavones and Flavonols against Herpes Simplex Virus Type-1 in Cell Culture. Comparison with the Antiviral Activity of Propolis. Journal of Natural Products, 55, 1732-1740.
https://doi.org/10.1021/np50090a003
[23]
Anonymous (1998) Commission on Dietary Supplement Labels Issues Final Report. Journal of the American Dietetic Association, 98, 270.
https://doi.org/10.1016/S0002-8223(98)00064-9
[24]
Anonymous (1997) FANSA Releases Statement about Dietary Supplement Labeling. Journal of the American Dietetic Association, 97, 728-729.
[25]
Anonymous (1997) First IND Submitted with FDA for an Herbal Pharmaceutical. AIDS Weekly Plus 18.
[26]
Ogundana, S.K., Coxon, D.T., Denis, C. and Nagavic, S.H.Z. (1980) Natural Antifugal Compounds from the Peel of Yam Tubers. Proceedings of the 6th Symposium of the TRC International Potato Center, 616.
[27]
Okigbo, R.N., Agbata, C.A. and Echezona, C.E. (2010) Effects of Leaf Extracts of Azadirachta indica and Chromolaena odorata on Post Harvest Spoilage of Yams in Storage. Current Research Journal of Biological Sciences, 2, 9-12.
[28]
Okigbo, R.N. and Nmeka, I.A. (2005) Control of Yam Tuber with Leaf of Xylopia aethiopica and Zingiber officinale. African Journal of Biotechnology, 4, 804-807.
[29]
Shiriki, D., Obochi, G.O., Eke, M.O. and Shambe, T. (2017) Postharvest Loss Control: Synergistic Plants Extract Inhibition of Ten Microbial Yam Rot Organisms. Food and Nutrition Sciences, 8, 725-732. https://doi.org/10.4236/fns.2017.87051
[30]
Amadioha, A.C. and Obi, V.I. (1999) Control of Anthracnose Disease of Cowpea by Cymbopogon citrates and Ocimum gratissimum. Acta Phytopathologica et Entomologica Hungarica, 34, 85-89.
[31]
Banso, A. and Sani, A. (2003) Antibacterial Effect of Leaf Extract of Ricinus communis. African Scientist, 4, 129-133.
[32]
Delahaye, C., Rainford, L., Nicholson, A., Mitchell, S., Lindo, J. and Ahmad, M. (2009) Antibacterial and Antifungal Analysis of Crude Extract from the Leaves of Callistemon viminalis. J. Med. And Biol. Scs. 3, 1-7.
[33]
Nagamani, J.E., Vidya, S.D. and Syeda, H.B. (2014) A Study on Antioxidant and Antimicrobial Properties of Bombax Ceiba pentandra Seed Extract. World Journal of Pharmacy and Pharmaceutical Sciences, 3, 692-706. http://www.wipps.com
[34]
Ezeibekwe, I.O. and Ibe, A.E. (2010) Fungal Organisms Associated with Yam (Dioscorea rotundata, poir) Rot at Owerri, Imo State of Nigeria. Journal of Molecular Genetics, 2, 1-5. https://doi.org/10.3923/jmolgene.2010.1.5
[35]
Okigbo, R.N. and Ikediugwu, F.E.O. (2000) Studies on Biological Control of Postharvest Rot of Yam (Diocorea spp.) with Trichoderma viride. Journal of Phytopathology, 148, 351-355. https://doi.org/10.1111/j.1439-0434.2000.tb04786.x
[36]
Tijjani, A. (2013) Antibiotic Synergy as a New Strategy for Combacting Drug Resistant Bacteria.
[37]
Wendakoon, C., Calderon, P. and Gagnon, D. (2012) Evaluation of Selected Medicinal Plants Extracted in Different Ethanol Concentrations for Antibacterial Activity against Human Pathogens. Journal of Medicinally Active Plants, 1, 60-68.
http://scholarworks.umass.edu/jmap/vol1/iss2/4
[38]
Akrayi1, H.F.S. (2013) Antibacterial Potency of Aqueous Plant Extracts against Streptococcus Mutans. Medical Journal of Islamic World Academy of Sciences, 22, 85-89. https://doi.org/10.12816/0008177
[39]
Irobi, O.N. (1997) Antibiotic Properties of Ethanol Extract of Chromolaena odorata (Asteriaceae). International Journal of Pharmacognosy, 35, 111-115.
https://doi.org/10.1076/phbi.35.2.111.13287
[40]
Azwanida, N.N. (2015) A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal and Aromatic Plants, 4, 3.
https://doi.org/10.4172/2167-0412.1000196
[41]
Czerwińska, E. and Szparaga, A. (2015) Antibacterial and Antifungal Activity of Plant Extracts. Annual Set the Environment Protection, 17, 209-229.
