In-vitro callus
induction and regeneration method was developed using different plant growth
regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and
Gamborg (B5) media) of Citrussinensis (L.)
Osbeck. Observations of the effect of PGRs were carried out using different
concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic
acid (NAA) and combinations of 2,4-D and NAA using different basal media. This
study found Citrussinensis (L.)
Osbeck exhibited a high frequency of callus induction on MS medium supplemented
with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6
and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9%
respectively
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