%0 Journal Article
%T <i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media
%A Md. Nazmul Hasan
%A Mohammed Raqibul Hasan
%A Shakhawat Hossain Foysal
%A Hammadul Hoque
%A Md. Fahim Khan
%A Md. Fahmid Hossain Bhuiyan
%A Shamsul H. Prodhan
%J American Journal of Plant Sciences
%P 285-297
%@ 2158-2750
%D 2019
%I Scientific Research Publishing
%R 10.4236/ajps.2019.102022
%X <i><span style=\"font-family:Verdana;\">&lt;i&gt;</span></i></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">In-vitro</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"> callus
induction and regeneration method was developed using different plant growth
regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and
Gamborg (B5) media) of </span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\">&lt;i&gt;</span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">Citrus</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"> <i>sinensis</i></span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"> (L.)
Osbeck. Observations of the effect of PGRs were carried out using different
concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic
acid (NAA) and combinations of 2,4-D and NAA using different basal media. This
study found </span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\">&lt;i&gt;</span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">Citrus</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"> <i>sinensis</i></span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"> (L.)
Osbeck exhibited a high frequency of callus induction on MS medium supplemented
with 3 mg/L 2,4-D and callus induction frequency was 86.7% ¡À 3.4% whereas N6
and B5 showed lower callus induction frequency of 83.3% ¡À 8.8% and 82.2% ¡À 1.9%
respectively
%K &lt
%K i&gt
%K Citrus sinensis&lt
%K /i&gt
%K (L.) Osbeck
%K PGRs
%K Basal Media
%K Regeneration
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=90750