优质能源甜高粱突变体的筛选与鉴定
Identification of sweet sorghum mutants for rich soluble sugars and high biomass enzymatic digestibility

以甜高粱品种能饲一号为野生型构建 EMS 化学诱变突变体库，通过分析突变体可溶性糖含量、生物质总量和生物质酶解产糖效率，观察农艺性状，并测定秸秆细胞壁结构组成，筛选得到 3 份特异突变体材料:SM83、SM197、SM305。3 份突变体除生物量皆显著提高以外，SM197 与 SM305 半纤维素含量显著升高，而木质素和纤维素含量明显降低，导致生物质酶解产糖效率大大提高。此外，SM83 的产糖效率与常规品种（对照）无显著差异，但其可溶性糖含量比野生型增加 14.99%。故筛选到的甜高粱突变体是理想的生物能源种质材料，可用于优质能源甜高粱品种的培育。
The large EMS mutagenesis pool of sweet sorghum was initially screened out．Three mutants were identified and designated as SM83,SM197 and SM305.In general,three mutants had high biomass productions,but displayed a distinct biomass enzymatic digestibility under chemical pretreatments.As a comparison,both SM197 and SM305 mutants were determined with much high biomass enzymatic saccharification,due to its high hemicelluloses levels and relatively low cellulose and lignin contents.Despite that SM83 mutant remained a similar biomass digestion rate,it contained an increased soluble sugar level by 14.99%,compared with wild type.Hence,the sweet sorghum mutants will be used for breeding energy sweet sorghum.