%0 Journal Article %T 优质能源甜高粱突变体的筛选与鉴定<br>Identification of sweet sorghum mutants for rich soluble sugars and high biomass enzymatic digestibility %A 张会 %A 邹维华 %A 张友兵 %A 张锐 %A 丰胜求 %A 涂媛苑 %A 景海春 %A 彭良才 %J 华中农业大学学报 %D 2015 %X 以甜高粱品种能饲一号为野生型构建 EMS 化学诱变突变体库,通过分析突变体可溶性糖含量、生物质总量和生物质酶解产糖效率,观察农艺性状,并测定秸秆细胞壁结构组成,筛选得到 3 份特异突变体材料:SM83、SM197、SM305。3 份突变体除生物量皆显著提高以外,SM197 与 SM305 半纤维素含量显著升高,而木质素和纤维素含量明显降低,导致生物质酶解产糖效率大大提高。此外,SM83 的产糖效率与常规品种(对照)无显著差异,但其可溶性糖含量比野生型增加 14.99%。故筛选到的甜高粱突变体是理想的生物能源种质材料,可用于优质能源甜高粱品种的培育。<br>The large EMS mutagenesis pool of sweet sorghum was initially screened out.Three mutants were identified and designated as SM83,SM197 and SM305.In general,three mutants had high biomass productions,but displayed a distinct biomass enzymatic digestibility under chemical pretreatments.As a comparison,both SM197 and SM305 mutants were determined with much high biomass enzymatic saccharification,due to its high hemicelluloses levels and relatively low cellulose and lignin contents.Despite that SM83 mutant remained a similar biomass digestion rate,it contained an increased soluble sugar level by 14.99%,compared with wild type.Hence,the sweet sorghum mutants will be used for breeding energy sweet sorghum. %K 甜高粱 EMS 突变体库 植物细胞壁 生物质降解 能源作物< %K br> %K sweet sorghum EMS mutagenesis pool plant cell wall biomass digestibility bioenergy crop %U http://hnxbl.cnjournals.net/hznydxzr/ch/reader/view_abstract.aspx?file_no=20150501&flag=1