鱼腥蓝细菌PCC 7120异形胞的分离
Isolating heterocysts from Anabaena sp. strain PCC 7120

鱼腥蓝细菌 PCC 7120是一种光合自养型丝状蓝细菌。缺氮条件下，菌丝上5% ~10%的营养细胞分化为异形胞，发挥固氮作用。异形胞是研究生物固氮和细胞分化的理想材料。为研究异形胞生理功能及其发育过程中的基因表达变化，需要从菌丝中分离高质量的异形胞。用2 mg/mL溶菌酶与0.1% Triton X-100同时处理菌丝30 min，得到了完整性好、纯度高的异形胞。
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 is able to undergo cell differentiation.When facing nitrogen deficiency,5%~10% vegetative cells will develop into heterocysts for fixing nitrogen.Thus this strain is an excellent material for studying nitrogen fixation and prokaryotic cell differentiation.Isolation of high quality heterocysts is a prerequisite to study their physiological changes and regulation of related gene.However,currently available methods for isolating heterocyst are usually time-consuming,low yield and low purity,unable to fullfill the requirements of many researches.An efficient method for isolating heterocyst was developed,in which vegetative cells were effectively broken in the presence of 2 mg/mL lysozyme and 0.1% Triton X-100,allowing heterocysts to be enriched with high purity and good integrity.This method will greatly facilitate heterocyst-related studies.