山西省欧李褐腐病病原菌鉴定
Pathogen Identification of Brown Rot of Cerasus humilis (Bge) Sok. in Shanxi

为明确山西省欧李褐腐病病原菌种类，采用组织分离法、致病性测定、形态学鉴定及rDNA-ITS序列测定对病原菌进行研究。结果显示，该菌在PDA培养基上菌落呈同心轮纹状，灰白或灰黄色，产孢丰富；分生孢子梗较短，有分枝；分生孢子串生，色，单胞，柠檬形或卵圆形，孢子大小为（7.5～20.0）μm×（7.5～11.5）μm。经形态学鉴定为美澳型核果链核盘菌(Monilinia fructicola)。rDNA-ITS序列与Monilinia fructicola的同源性为100%，分子鉴定与形态学鉴定结果一致。
The pathogen of Cerasus humilis (Bge) Sok. brown rot in Shanxi province was isolated and identified by tissue isolation,pathogenicity test,morphological characters and rDNA-ITS sequence analysis. The colony of isolate was grey,concentric rings and sporulation were abundant. Conidiophores were short and branched. Conidia were in chain,hyaline,unicellular,lemon-shaped or ovate,(7.5-20.0) μm×(7.5-11.5) μm. The results showed that the pathogen was Monilinia fructicola. rDNA-ITS sequence showed 100% identity with Monilinia fructicola. The molecular identification indicated the same results as morphology identification.