%0 Journal Article
%T 山西省欧李褐腐病病原菌鉴定<br>Pathogen Identification of Brown Rot of Cerasus humilis (Bge) Sok. in Shanxi
%A 郝晓娟
%A 周
%A 芳
%A 李新凤
%A 王美琴
%A 王建明
%A 李俊林
%J 西北农业学报
%D 2015
%R 10.7606/j.issn.1004-1389.2015.04.017
%X 为明确山西省欧李褐腐病病原菌种类，采用组织分离法、致病性测定、形态学鉴定及rDNA-ITS序列测定对病原菌进行研究。结果显示，该菌在PDA培养基上菌落呈同心轮纹状，灰白或灰黄色，产孢丰富；分生孢子梗较短，有分枝；分生孢子串生，色，单胞，柠檬形或卵圆形，孢子大小为（7.5～20.0）μm×（7.5～11.5）μm。经形态学鉴定为美澳型核果链核盘菌(Monilinia fructicola)。rDNA-ITS序列与Monilinia fructicola的同源性为100%，分子鉴定与形态学鉴定结果一致。<br>The pathogen of Cerasus humilis (Bge) Sok. brown rot in Shanxi province was isolated and identified by tissue isolation,pathogenicity test,morphological characters and rDNA-ITS sequence analysis. The colony of isolate was grey,concentric rings and sporulation were abundant. Conidiophores were short and branched. Conidia were in chain,hyaline,unicellular,lemon-shaped or ovate,(7.5-20.0) μm×(7.5-11.5) μm. The results showed that the pathogen was Monilinia fructicola. rDNA-ITS sequence showed 100% identity with Monilinia fructicola. The molecular identification indicated the same results as morphology identification.
%K 欧李 褐腐病 病原菌鉴定 美澳型核果链核盘菌&lt
%K br&gt
%K Cerasus humilis (Bge) Sok. Brown rot Pathogen identification Monilinia fructicola
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20150417&flag=1