特发性矮小患儿血中p53及p21waf/cip1的表达*
Expressions of p53 and p21waf/cip1 in blood of children with idiopathic short stature

目的：观察特发性矮小（ISS）患儿血中p53及细胞周期调节基因p21waf/cip1的表达，探讨二者与ISS发病机制之间的关系。方法：采用实时荧光定量PCR及ELISA法检测30例ISS患儿（ISS组）和30名正常儿童（对照组）外周血中p53和p21waf/cip1 mRNA的表达水平及血清中P53和P21waf/cip1蛋白的含量。结果：ISS组患儿外周血中p53 mRNA的表达水平及血清P53的浓度与对照组相比，差异无统计学意义（t=1.044和0.701，P均＞0.05），ISS组p21waf/cip1 mRNA的表达水平较对照组升高（t=4.606，P<0.001）。结论：p21waf/cip1可能通过非依赖p53途径参与ISS的发生。
Aim: To investigate the expressions of p53 and p21waf/cip1 in blood of children with idiopathic short stature(ISS) and to elucidate the pathogenesis of ISS. Methods: Real？？time PCR was used to measure the expressions of p53 mRNA and p21waf/cip1 mRNA in peripheral blood from 30 children with ISS(ISS group) and 30 normal control(control group); ELISA was used to measure the levels of P53 and P21waf/cip1 in serum of the children in the 2 groups. Results: The expression of p53 mRNA and the concentration of serum P53 had no statistical significance between the 2 groups(t=1.044 and 0.701，P＞0.05）. The expression of p21waf/cip1 mRNA in ISS group was significantly higher than those in the control group(t=4.066，P<0.001). Conclusion: p21waf/cip1 may be responsible for the pathogenesis of ISS, which might not depends on p53 pathway