慢性髓性白血病患者骨髓中Notch信号通路的表达*
Expression of Notch signal pathway in bone marrow from patients with chronic myeliod leukaemia

目的:探讨慢性髓性白血病(CML)患者骨髓中Notch信号通路的表达水平及临床意义。方法:采用实时荧光定量PCR检测24例CML患者(慢性期12例、急变期12例)和9例正常对照骨髓中Notch1、Notch2、 Delta-like1、Jagged1及Hes1 mRNA的表达。结果:急变期CML患者骨髓中Notch1和Notch2 mRNA表达水平高于正常对照组及慢性期患者(P＜0.05),Delta-like1 mRNA表达水平低于正常对照组及慢性期患者(P＜0.05)。慢性期及急变期患者骨髓中Jagged1、Hes1 mRNA表达水平高于正常对照组(P＜0.05)。Notch1、Notch2、Jagged1、Hes1 mRNA表达水平与CML患者临床特征无关(P＞0.05),仅染色体异常的CML患者Delta-like1 mRNA表达水平较染色体正常者升高(P＜0.05)。结论:Notch信号通路可能参与了CML的发病和急性变。
Aim: To explore the expression of Notch signal pathway in bone marrow from patients with chronic myeliod leukaemia(CML).Methods: Bone marrow from 24 cases of CML(including 12 cases of chronic phase,12 cases of blast crisis)and 9 subjects as control were collected to detect mRNA expression of Notch1,Notch2,Delta-like1,Jagged1,and Hes1 using RT-qPCR.Results: The expression levels of Notch1 and Notch2 mRNA in blast crisis patients were both significantly higher than those in chronic phase patients and the control group(P＜0.05),while the expression level of Delta-like1 mRNA was lower(P＜0.05).The expression level of Jagged1 and Hes1 mRNA in chronic phase and blast crisis patients were significantly higher than those in the control group(P＜0.05).The expression levels of Notch1,Notch2,Jagged1,and Hes1 mRNA in CML patients did not related to clinical parameters(P＞0.05),but Delta-like1 mRNA expressed highly in patients with abnormal karyotype(P＜0.05).Conclusion: Notch signal pathway may play roles in the pathogenesis and blast crisis of CML