miR？？GAAB2？？200a与金雀异黄酮对胃癌细胞生长及侵袭能力的影响
Synergistic effect of miR？？GAAB2？？200a and genistein on growth and invasion of gastric cancer cells

目的:探讨miR？？GAAB2？？200a与金雀异黄酮对3种胃癌细胞生长及侵袭能力的影响及可能机制。方法:MGC803、BGC823、MKN45细胞分别分为4组:阴性对照组(转染阴性对照NC mimic)、金雀异黄酮组(加入50 μmol/L的金雀异黄酮)、miR？？GAAB2？？200a组(转染miR？？GAAB2？？200a mimic)、联合组(miR？？GAAB2？？200a mimic转染联合50 μmol/L的金雀异黄酮处理),MTT法观察处理24 h对细胞生长的影响,Transwell小室实验观察细胞侵袭能力的变化,RT？？GAAB2？？PCR及Western blot检测各组细胞EphA2 mRNA及蛋白的表达。结果:miR？？GAAB2？？200a与金雀异黄酮单用均可抑制部分胃癌细胞的生长、侵袭及EphA2蛋白的表达,两者交互作用仅出现在BGC823细胞中(P<0.05)。结论:miR？？GAAB2？？200a与金雀异黄酮可能通过调控EphA2抑制胃癌细胞生长及侵袭,且二者存在部分协同作用。
Aim: To investigate the effects and possible mechanisms of miR？？GAAB2？？200a and genistein on the growth and invasion of gastric cancer cells.Methods: The human gastric cancer cell lines MGC803, BGC823 and MKN45 were used as research objects. The cells were divided into 4 groups,negative control group(transfected with NC mimic),genistein group(treated by 50 μmol/L genistein),miR？？GAAB2？？200a group(transfected with miR？？GAAB2？？200a mimic),and combination group(transfected with miR？？GAAB2？？200a mimic and treated by 50 μmol/L genistein). The effects of miR？？GAAB2？？200a and genistein on the growth of gastric cancer cells were observed by MTT assay.Transwell chamber was used to verify the effect on cell invasion. RT-PCR and Western blot were used to observe the effects of miR？？GAAB2？？200a and genistein on EphA2 mRNA and protein.Results: Both miR？？GAAB2？？200a and genistein could inhibit the growth and invasion of part of the gastric cancer cells and inhibit the expression of EphA2 protein, and the synergistic effects of miR-200a and genistein were only found in BGC823 cells(P<0.05).Conclusion: miR？？GAAB2？？200a and genistein might inhibit the growth and invasion of gastric cancer cells through down-regulation of EphA2,and there are some synergistic effects