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- 2016
18β-甘草次酸哌嗪衍生物抗肝癌SMMC-7721细胞活性研究
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Abstract:
摘要：目的 研究18β-甘草次酸哌嗪衍生物D34、D35对SMMC-7721细胞增殖的的抑制作用及对凋亡的影响。方法 用18β-甘草次酸哌嗪衍生物D34、D35以不同浓度、不同时间段处理SMMC-7721细胞，显微镜下观察其对肝癌SMMC-7721细胞形态结构的影响；MTT法检测18β-甘草次酸哌嗪衍生物D34、D35对SMMC-7721细胞的增殖抑制率；Hoechst-33258细胞凋亡染色试剂盒检测细胞凋亡情况；Annexin V-FITC/PI双染法检测细胞凋亡率。结果 MTT检测结果表明，18β-甘草次酸哌嗪衍生物D34、D35能够抑制肝癌SMMC-7721细胞增殖，且呈浓度依赖性(P<0.05)；流式细胞仪检测细胞凋亡率，D35为26.71%、D34为36.95%。结论 18β-甘草次酸哌嗪衍生物D34、D35对SMMC-7721细胞有增殖抑制作用和促凋亡作用，且优于18β-甘草次酸(GA)。
ABSTRACT: Objective To study the effects of 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 on the proliferation and apoptosis of SMMC-7721cells. Methods The morphology of the cells treated with 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 was observed under the microscope. The cellular inhibition rate was detected by MTT; apoptosis was detected by Hoechst-33258 Staining Kit; and apoptosis rate was analyzed by flow cytometry method. Results The proliferation of SMMC-7721 cells was significantly inhibited by 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 in a dose-dependent manner (P<0.05). Conclusion 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 can inhibit the proliferation and induce the apoptosis of SMMC-7721 cells, and they are superior to GA
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