%0 Journal Article %T 18β-甘草次酸哌嗪衍生物抗肝癌SMMC-7721细胞活性研究<br>Effects of 18β-glycyrrhetinic acid derivatives containing piperazine on SMMC-7721 cell activity %A 郝秀静 %A 阳 %A 辉 %A 王 %A 娟 %A 李 %A 敏 %A 李学强 %J 西安交通大学学报(医学版) %D 2016 %R 10.7652/jdyxb201606005 %X 摘要:目的 研究18β-甘草次酸哌嗪衍生物D34、D35对SMMC-7721细胞增殖的的抑制作用及对凋亡的影响。方法 用18β-甘草次酸哌嗪衍生物D34、D35以不同浓度、不同时间段处理SMMC-7721细胞,显微镜下观察其对肝癌SMMC-7721细胞形态结构的影响;MTT法检测18β-甘草次酸哌嗪衍生物D34、D35对SMMC-7721细胞的增殖抑制率;Hoechst-33258细胞凋亡染色试剂盒检测细胞凋亡情况;Annexin V-FITC/PI双染法检测细胞凋亡率。结果 MTT检测结果表明,18β-甘草次酸哌嗪衍生物D34、D35能够抑制肝癌SMMC-7721细胞增殖,且呈浓度依赖性(P<0.05);流式细胞仪检测细胞凋亡率,D35为26.71%、D34为36.95%。结论 18β-甘草次酸哌嗪衍生物D34、D35对SMMC-7721细胞有增殖抑制作用和促凋亡作用,且优于18β-甘草次酸(GA)。<br>ABSTRACT: Objective To study the effects of 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 on the proliferation and apoptosis of SMMC-7721cells. Methods The morphology of the cells treated with 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 was observed under the microscope. The cellular inhibition rate was detected by MTT; apoptosis was detected by Hoechst-33258 Staining Kit; and apoptosis rate was analyzed by flow cytometry method. Results The proliferation of SMMC-7721 cells was significantly inhibited by 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 in a dose-dependent manner (P<0.05). Conclusion 18β-glycyrrhetinic acid derivatives containing piperazine D34 and D35 can inhibit the proliferation and induce the apoptosis of SMMC-7721 cells, and they are superior to GA %K 18β-甘草次酸 %K 哌嗪衍生物 %K SMMC-7721 %K 细胞凋亡< %K br> %K 18β-glycyrrhetinic acid %K piperazine derivative %K SMMC-7721 %K apoptosis %U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201606005