|
|
- 2016
髓样细胞触发受体-1在寻常型银屑病中的表达及意义
|
Abstract:
摘要：目的 通过对比髓样细胞触发受体-1(Trem-1)在寻常型银屑病患者与正常人皮肤组织及血液中的表达差异，探索寻常型银屑病发病的可能机制。方法 采用免疫组化及实时荧光定量PCR的方法检测Trem-1在不同组织中的表达情况，应用SPSS21.0统计软件进行统计学分析。结果 Trem-1在寻常型银屑病患者皮损中表达增加，主要表达于表皮全层，正常皮肤组织中主要表达于基底细胞层，其表达差异具有统计学意义(P<0.05)。Trem-1 mRNA在寻常型银屑病皮损、血液中表达升高，与正常组相比差异具有统计学意义(P<0.05)；且Trem-1mRNA表达与银屑病皮损面积和严重程度指数(PASI)呈正相关(P<0.05)。结论 Trem-1的异常表达可能参与了寻常型银屑病发病，Trem-1可能成为治疗寻常型银屑病的潜在靶点。
ABSTRACT: Objective To study the expression of triggering receptor expressed on myeloid cells-1 (Trem-1) in psoriatic vulgaris and normal skin tissues and blood, and to explore the potential pathogenesis of psoriasis. Methods Immunohistochemistry and Real-time PCR were used to detect the expression of Trem-1 in the blood and tissues of normal skin and psoriasis. Results The positive expression rate of Trem-1 in psoriatic lesion was significantly higher than normal tissue. Trem-1 was expressed in the whole epidermis, with a significant difference (P<0.05). The mRNA expression of Trem-1 was significantly higher in psoriatic skin tissues and blood than in normal skin tissues and blood (P<0.05). Moreover, the mRNA expression of Trem-1 was positively correlated with PASI (P<0.05). Conclusion Abnormal expression of Trem-1 might be related to the pathogenesis of psoriasis. Trem-1 will cure psoriasis vulgaris as the potential therapeutic target
| [1] | KOUSSOULAS V, VASSILIOU S, DEMONAKOU M, et al. Soluble triggering receptor exposed on myeloid cells(sTREM-1): a new mediator involved in the pathogenesis of peptic ulcer disease[J]. Eur J Gastroenterol Hepatol, 2006, 18(4):375-379. |
| [2] | MURAKAMI Y, AKAHOSHI T, AOKI N, et al. Intervention of inflammation amplifier, triggering receptor expressed on myeloid ceils l, for treatment of autoimmune arthritis[J]. Arthritis Rheum, 2009, 60(6):1615-1623. |
| [3] | 王晓华. 髓系细胞触发受体-1在疾病中的研究进展[J]. 南通大学学报, 2012, 32(3):217-220. |
| [4] | BRZEWSKI PL, SPALKOWSKA M, PODBIELSKA M, et al. The role of focal infections in the pathogenesis of psoriasis and chronic urticaria[J]. Postepy Dermatol Alergol, 2013, 30(2):77-84. |
| [5] | BOSTANCIN, THURNHEER T, BELIBASAKIS GN, et al. Involvement of the TREM-1/DAP12 pathway in the innate immune responses to Porphyromonas gingivalis [J]. Mol Immunol, 2011, 49(1-2):387-394. |
| [6] | TESEAN AS, CERWENKA A. The TREM-1/DAPl2 pathway[J]. Immunol Left, 2008, 116(2):111-116. |
| [7] | TESTATE AS, WEILER S, ZANZINGER K, et al. Non-T cell activation linker (NTAL) negatively regulates TREM-I/DAPl2-induced inflammatory cytokine production in myeloid cells[J]. J lmmunol, 2007, 178(4):199l-1999. |
| [8] | G？MEZ-PI？A V, MART？PNEZ E, FERN？BNDEZ-RU？PZ I, et al. Role of MMPs in orchestrating inflammatory response in human monocytes via a TREM-1-PI3K-NF-κB pathway[J]. J Leukoc Biol, 2012, 91(6):933-945. |
| [9] | BLCHARSKI JR, KIESSLER V, BUONSANTI C, et al. A role for triggering receptor expressed on myeloid cells-1 in host defense during the early induced and adaptive phases of the immune response[J]. J Immunol, 2003, 170(7):3812-3818. |
| [10] | HYDER LA, GONZALEZ J, HARDEN JL, et al. TREM-1 as a potential therapeutic target in psoriasis [J]. J Invest Dermatol, 2013, 133(7):1742-1751. |
| [11] | WANG DY, QIN RY, LIU ZR, et al. Expression of TREM-1 mRNA in acute pancreatitis [J]. World J Gastroenterol, 2004, 10(18):2744-2746. |
| [12] | WU M, PENG A, SUN M, et al. TREM-1 amplifies comeal inflammation after Pseudomonas aeruginosa infection by modulating Toll-like receptor signaling and Th1/Th2-type immune response[J]. Nat Med, 2001, 79(7):2709-2716. |
| [13] | BOUCHON A, DIETRICH J, COLONNA M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes[J]. J Immunol, 2000, 164(10):4991-4995. |
