|
|
- 2016
CD31+/CD42b-、CD144+内皮微粒水平与颈动脉内膜中层厚度的关系
|
Abstract:
摘要：目的 检测血浆中CD31+/CD42b-、CD144+内皮微粒水平，探讨其与颈动脉内膜中层厚度及动脉粥样硬化危险因素间的关系。方法 选取我院心内科门诊及住院患者122例，行颈动脉超声检查，根据超声结果分为增厚组(62例)和对照组(60例)；采用流式细胞仪检测2组患者血浆中CD31+/CD42b-、CD144+内皮微粒水平，分析其与颈动脉内膜中层厚度、动脉粥样硬化危险因素的相关性。结果 与对照组比较，增厚组CD31+/CD42b-、CD144+内皮微粒水平明显升高；血浆中CD31+/CD42b-、CD144+内皮微粒水平与颈动脉内膜中层厚度呈正相关；多因素Logistic回归分析显示，CD31+/CD42b-内皮微粒水平与年龄、TC和Cys C呈正相关；血浆CD144+内皮微粒水平与UA和BMI呈正相关。结论 血浆中的CD31+/CD42b-、CD144+内皮微粒水平与颈动脉内膜厚度相关，对心血管疾病的早期预防具有指导意义。
ABSTRACT: Objective To detect the levels of CD31+/CD42b- and CD144+ endothelial microparticles (EMPs) in plasma, and analyze the relationship with carotid intima-media thickness (CIMT) and the risk factors of atherosclerosis. Methods Totally 122 outpatients and inpatients in our hospital were selected, and carotid artery ultrasound examination was performed. The results were divided into a thickening group (62 cases) and a control group (60 cases). Flow cytometry was used to detect the levels of CD31+/CD42b- and CD144+ EMPs in the blood to analyze the relationship between the level of endothelial cells and CIMT, and to investigate the relationship between the level of plasma EMPs and the risk factors of atherosclerosis. Results Compared with the control group, the levels of EMPs of CD31+/CD42b- and CD144+ were significantly higher. In the correlation analysis, plasma CD31+/CD42b- and CD144+EMPs levels were positively correlated with CIMT; in multi-factor Logistic regression analysis, CD31+/CD42b- EMPs levels were correlated positively with age, TC and Cys C; plasma CD144+ EMPs levels were correlated with UA and BMI. Conclusion The levels of CD31+/CD42b-, CD144+ EMPs in plasma were correlated with CIMT, guiding early prevention of cardiovascular diseases
| [1] | RAUTOU PE, VION AC, AMABILE N, et al. Microparticles, vascular function, and atherothrombosis[J]. Citv Res, 2011, 109(5):593-606. |
| [2] | HU SS, ZHANG HG, ZHANG QJ, et al. Small-size circulating endothelial microparticles in coronary artery disease[J]. Plos One, 2014, 9(8):104. |
| [3] | DE RANGO P. Commentary on: predicting carotid artery disease and plaque instability from cell-derived microparticles[J]. Eur J Vasc Endovasc Surg, 2014, 48(5):496-497. |
| [4] | SORIANO S, CARMONA A, TRIVINO F, et al. Endothelial damage and vascular calcification in patients with chronic kidney disease[J]. Am J Phy, 2014, 307(11):1302-1311. |
| [5] | 黄宇理，李秒男，徐玮，等. 瑞舒伐他汀对老年急性冠脉综合征患者血浆内皮微粒的影响[J]. 中国老年学杂志, 2015, 35(4):2027-2028. |
| [6] | 李莹，夏金盈，陆英，等. 冠状动脉性心脏病患者外周血中CD144<sup>+</sup>/Annexin V<sup>+</sup>内皮微颗粒水平的变化[J]. 上海医学, 2012, 35(1):53-57. |
| [7] | BRODSKY SV, ZHANG F, NASJLETTI A, et al. Endothelium-derived microparticles impair endothelial function in vitro[J]. Am J Physiol Heart Circ Physiol, 2004, 286(5):1910-1915. |
| [8] | NOZAKI T, SUGIYAMA S, KOGA H, et al. Significance of a multiple biomarker strategy including endothelial dysfunction to improve risk stratification for cardiovascular events in patients at high risk for coronary heart disease[J]. J Am Coll Cardiol, 2009, 54(2):601-608. |
| [9] | BLANN A, SHANTSILA E, SHANTSILA A. Microparticles and arterial disease [J]. Semin Thromb Hemost, 2009, 35(5):488-496. |
| [10] | ROBERTSON CM, GERRY F, FOWKES R, et al. Carotid thickness and the prediction of intima-media vascular events[J]. Vasc Med, 2012, 17:239-248. |
| [11] | CHEN Y, FENG B, LI X, et al. Plasma endothelial microparticles and their correlation with the presence of hypertension and arterial stiffness in patients with type 2 diabetes [J]. Clin Hypertens, 2012, 14(7):455-460. |
| [12] | GE Y, CHENG S,LARSON MG, et al. Circulating CD31<sup>+</sup> leukocyte frequency is associated with cardiovascular risk factors[J]. Atherosclerosis, 2013, 229(1):228-233. |
| [13] | DIGNAT-GEORGE F, BOULANGER CM. The many faces of endothelial microparticles[J]. Arterioscler Thromb Vasc Biol, 2011, 31(1):27-33. |
| [14] | WEKESA AL, CROSS KS, O？？DONOCAN O, et al. Predicting carotid artery disease and plaque instability from cell-derived microparticles [J]. Eur J Vasc, 2014, 48(5):489-495. |
| [15] | NARIN N, YILMAZ E, PAMUKCU O, et al. Are endothelial microparticles early markers of pulmonary hypertension [J]. Biomarkers, 2014,19(4):319-325. |
| [16] | 田刚，任燕，卢群，等. 高糖诱导人脐静脉内皮细胞内皮微粒释放并促进细胞凋亡[J]. 西安交通大学学报(医学版), 2010, 31(2):181-185. |
| [17] | 马鸿伟，任卫东，张斌，等. 阿托伐他汀对2型糖尿病患者内皮细胞微粒和颈动脉内膜中膜厚度的影响[J]. 中国动脉硬化杂志, 2015, 23(8):829-833. |
