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- 2017
柠檬酚对人肝癌HepG2细胞增殖的抑制作用及其机制
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Abstract:
摘要：目的 研究黄酮类化合物柠檬酚对人肝癌HepG2细胞增殖的抑制作用及其机制。方法 采用离体培养的HepG2细胞为研究对象，以MTT法检测细胞增殖抑制率；以HE染色和吖啶橙染色法观察细胞形态及分布；利用流式细胞仪对HepG2细胞的分裂周期进行检测；以荧光显色法检测蛋白表达。结果 柠檬酚能够剂量依赖性地抑制HepG2细胞的增殖，其抑制作用的IC50值为76.46μmol/L。柠檬酚能够使HepG2细胞发生皱缩，将细胞分裂阻滞在G2/M期，并抑制细胞骨架蛋白F-actin的表达。结论 柠檬酚具有抑制肝癌HepG2细胞分裂周期、抑制HepG2细胞骨架形成的作用，进而具有抑制肝癌HepG2细胞增殖的作用。
ABSTRACT: Objective To study the inhibitory effect and mechanism of citrusinol on proliferation of human hepatocellular carcinoma cells HepG2. Methods The inhibitory rate of HepG2 cells cultured ？？ in vitro？？ was measured by MTT assay. The morphology and distribution of the cells were observed by HE and acridine orange staining. The cell division cycle was detected by flow cytometry. The expression of F-actin protein was observed by fluorescent chromogenic method. Results Citrusinol could inhibit the growth of HepG2 cells, and the IC？？？？50？？ of the inhibitory rate was 76.46μmol/L. Citrusinol could make the HepG2 cells shrink, arrest the cell division cycle to G2/M, and inhibit the expression of F-actin. Conclusion Citrusinol can prevent cell proliferation by arresting cell division cycle in G2/M phase and inhibiting the formation of cytoskeleton, thus inhibiting the growth of G2/M
| [1] | 庞晓斌，谢欣梅，赵清辉. 红景天苷对人肝癌HepG2细胞增殖及凋亡的影响[J]. 中国药学杂志, 2014, 49(3):195-198. |
| [2] | SHARMA P, PRAKSSH O, SHUKLA A, et al. Structure-activity relationship studies on holy basil (Ocimum sanctum L.) based flavonoid orientin and its analogue for cytotoxic activity in liver cancer cell line HepG2[J]. Comb Chem High Throughput Screen, 2016, 19(8):656-666. |
| [3] | FENG CP, TANG HM, HUANG S, et al. Evaluation of the effects of the water-soluble total flavonoids from Isodon lophanthoides var.gerardianus (Benth.) H. Hara on apoptosis in HepG2 cell: Investigation of the most relevant mechanisms[J]. J Ethnopharmacol, 2016, 188:70-79. |
| [4] | 陆国寿，叶勇，卢文杰，等. 壮、瑶药小槐花中异柠檬酚的分离鉴定及含量测定研究[J]. 天然产物研究与开发, 2014, 26(9):1450-1453. |
| [5] | 李海霞，马英丽，史灵恩，等. 蓬子菜黄酮类化合物对人肝癌HepG2细胞增殖及凋亡的机制研究[J]. 中草药, 2013, 44(10):1290-1294. |
| [6] | 黄岚珍，王娟，卢菲婷，等. 莪术醇抑制人肝癌细胞HepG2增殖的机制[J]. 中国中药杂志, 2013, 38(11):1812-1815. |
| [7] | 朱丹，王帝，王光辉，等. 小槐花大极性部位的化学成分研究[J]. 中国中药杂志, 2014, 39(16):3112-3116. |
| [8] | 刘超，王俊霞，顾雪竹，等. 小槐花提取物对α-葡萄糖苷酶抑制活性研究[J]. 中国实验方剂学杂志, 2013, 19(20):91-93. |
| [9] | LI YQ, YANG F, WANG L, et al. Phosphoramidate protides of five flavones and their antiproliferative activity against HepG2 and L-O2 cell lines[J]. Eur J Med Chem, 2016, 112:196-208. |
| [10] | HO GT, KASE ET, WANGENSTEEN H, et al. Effect of phenolic compounds from elderflowers on glucose and fatty acid uptake in human myotubes and HepG2-cells[J]. Molecules, 2017, 22(1):90-96. |
| [11] | GUO H, REN F, ZHANG L, et al. Kaempferol induces apoptosis in HepG2 cells via activation of the endoplasmic reticulum stress pathway[J]. Mol Med Rep, 2016, 13(3):2791-2800. |
