投喂频率对流水养殖鞍带石斑鱼生长、摄食及免疫酶活力的影响
The Effect of Feeding Frequency on Growth Performance, Food Intake and Immune Enzyme Activities of Epinephelus lanceolatus in Flowing Water Cultivation

在水温（28.59±1.74）℃下，选用冰鲜杂鱼饲养平均体质量为858.73 g的鞍带石斑鱼，经过60 d的生长实验，研究了不同投喂频率（1次/d、1次/2 d、1次/3 d）对流水养殖鞍带石斑鱼生长、摄食及免疫酶活力的影响.结果表明，投喂频率对鞍带石斑鱼存活率的影响差异不具有统计学意义（p > 0.05），实验期间各个实验组存活率在99.40%~99.80%之间；各实验组间的肥满度（CF=2.22~2.35）、体质量变异系数（SV=20.08~24.27）、饲料系数（FCR=2.70~3.01）和日增质量（7.50~12.51 g/d）均无显著影响（p > 0.05），而投喂频率1次/d组和1次/2 d组的特定生长率（SGR）和相对增质量（RWG）均显著高于1次/3 d组（p < 0.05），投喂频率1次/d组的日摄食率和1次/2 d组的饲料转化效率均显著高于1次/3 d组（p < 0.05）；在免疫酶活力方面，投喂频率对鞍带石斑鱼血浆中的碱性磷酸酶（AKP=0.083 5~0.089金氏单位/mL）、过氧化氢酶（CAT=7.96~8.19 U/mL）及超氧化物歧化酶（SOD=32.40~34.45 U/mL）均无显著影响（p > 0.05）.在本实验条件下，流水模式养殖鞍带石斑鱼的适宜投喂频率为1次/d或1次/2 d.
A 60-day experiment was conducted to determine the effects of different feeding frequencies (once per day, once every 2 days and once every 3 days) on the growth performance and immune enzyme activities of Epinephelus lanceolatus with an average weight of 858.73 g at water temperature (28.59±1.74℃), reared with raw fish feed in a flowing water system. The results showed that the survival rate of the fish was not significantly different among the three groups (p > 0.05), and the survival rate in all treatments was between 99.40% and 99.80% during the experiment. There were no significant effects on the condition factor (CF=2.22-2.35), coefficient of size variation (SV=20.08-24.27), food conversion ratio (FCR=2.70-3.01) and daily weight gain (7.50-12.51g/d) in all groups (p > 0.05). The specific growth rate (SGR) and relative weight gain (RWG) in the fish fed once per day and once every 2 days were both significantly higher than those of the fish fed once every 3 days (p < 0.05). Moreover, the food intake (FI) of the fish fed once per day was significant higher than that of once every 3 days (p < 0.05) and food conversion efficiency (FCE) in the fish fed once every 2 days was significantly higher that of the fish fed once every 3 days (p < 0.05). The immune enzyme activity (i.e. AKP=8.083 5-0.089 4 King/mL, CAT=7.96-8.19 U/mL and SOD=32.40-34.45 U/mL) of the fish was also not significant different in the three treatments (p > 0.05). Under the conditions of this experiment, the optimal feeding frequency was found at feeding frequency of once per day or once every two days in flowing water cultivation