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- 2017
梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管舒缩功能异常的改善作用
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Abstract:
探讨梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管舒缩功能异常的改善作用.用线栓法复制大鼠缺血/再灌注模型，分别缺血2 h，于再灌注1 d，4 d，7 d和14 d给药后，取脑，按照相应试剂盒测定脑组织NOS，ET-1，TXB2和6-Ke-to-PGF1α质量分数.结果表明，与模型组相比，再灌注4 d，7 d和14 d，梓葛冻干粉针16.40 mg/kg，32.70 mg/kg和65.40 mg/kg能显著降低TNOS，iNOS活力，ET-1质量分数，TXB2质量分数及TXB2/6-Ke-to-PGF1α的比值(p＜0.01)，且也能显著升高cNOS活力和6-Ke-to-PGF1α质量分数(p＜0.01).得出梓葛冻干粉针有改善脑缺血/再灌注损伤大鼠微血管舒缩功能异常的作用.
Objective To investigate the protective effect of Zige lyophilized powder for injection against the microvascular contraction function after cerebral ischemia-reperfusion injury of rats. Methods It made a cerebral ischemia-reperfusion model using the line in the left middle cerebral artery occlusion (MCAO) of rats in ischemia for 2 h and reperfusion for l d, 4 d, 7 d and 14 d, the model of rats was established by intravenous injection of drugs. Nitric oxide synthase (NOS), endothelin-1(ET-1), 6-ketone-prostaglandin F1α (6-Ke-to-PG-F1α) and thromboxane B2 (TXB2) content of the brain microvessel in the lateral ischemic cerebral cortex was detected by enzyme immunoassay kits. Results In reperfusion for 4, 7 and 14 d, compared with model group, Zige lyophilized powder groups (16.40 mg/kg, 32.70 mg/kg and 65.40 mg/kg) had reduced significantly the level of TNOS, iNOS, ET-1, TXB2 and TXB2/6-Ke-to-PGF1α in microvessel of the lateral ischemic cerebral cortex (p < 0.01), and had increased significantly the level of significantly cNOS and 6-Ke-to-PGF1α(p < 0.01). Conclusion Zige lyophilized powder for injection can improve the effect of cerebral microcirculation. Zige lyophilized powder can protect cerebral ischemia-reperfusion injury by improving the microvascular contraction function
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