%0 Journal Article
%T 梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管舒缩功能异常的改善作用<br>The Protective Effect of Zige Lyophilized Powder for Injection Against the Microvascular Contraction Function After Cerebral Ischemia-Reperfusion Injury of Rats in the Microcirculation
%A 尚远宏
%A 田金凤
%A 刘庆
%A 王敏
%A 徐晓玉&lt
%A br&gt
%A SHANG Yuan-hong
%A TIAN Jin-feng
%A LIU Qing
%A WANG Min
%A XU Xiao-yu
%J 西南大学学报(自然科学版)
%D 2017
%R 10.13718/j.cnki.xdzk.2017.06.004
%X 探讨梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管舒缩功能异常的改善作用.用线栓法复制大鼠缺血/再灌注模型，分别缺血2 h，于再灌注1 d，4 d，7 d和14 d给药后，取脑，按照相应试剂盒测定脑组织NOS，ET-1，TXB<sub>2</sub>和6-Ke-to-PGF<sub>1α</sub>质量分数.结果表明，与模型组相比，再灌注4 d，7 d和14 d，梓葛冻干粉针16.40 mg/kg，32.70 mg/kg和65.40 mg/kg能显著降低TNOS，iNOS活力，ET-1质量分数，TXB<sub>2</sub>质量分数及TXB<sub>2</sub>/6-Ke-to-PGF<sub>1α</sub>的比值(<i>p</i>＜0.01)，且也能显著升高cNOS活力和6-Ke-to-PGF<sub>1α</sub>质量分数(<i>p</i>＜0.01).得出梓葛冻干粉针有改善脑缺血/再灌注损伤大鼠微血管舒缩功能异常的作用.<br>Objective To investigate the protective effect of Zige lyophilized powder for injection against the microvascular contraction function after cerebral ischemia-reperfusion injury of rats. Methods It made a cerebral ischemia-reperfusion model using the line in the left middle cerebral artery occlusion (MCAO) of rats in ischemia for 2 h and reperfusion for l d, 4 d, 7 d and 14 d, the model of rats was established by intravenous injection of drugs. Nitric oxide synthase (NOS), endothelin-1(ET-1), 6-ketone-prostaglandin F<sub>1α</sub> (6-Ke-to-PG-F<sub>1α</sub>) and thromboxane B2 (TXB<sub>2</sub>) content of the brain microvessel in the lateral ischemic cerebral cortex was detected by enzyme immunoassay kits. Results In reperfusion for 4, 7 and 14 d, compared with model group, Zige lyophilized powder groups (16.40 mg/kg, 32.70 mg/kg and 65.40 mg/kg) had reduced significantly the level of TNOS, iNOS, ET-1, TXB<sub>2</sub> and TXB<sub>2</sub>/6-Ke-to-PGF<sub>1α</sub> in microvessel of the lateral ischemic cerebral cortex (<i>p</i> < 0.01), and had increased significantly the level of significantly cNOS and 6-Ke-to-PGF<sub>1α</sub>(<i>p</i> < 0.01). Conclusion Zige lyophilized powder for injection can improve the effect of cerebral microcirculation. Zige lyophilized powder can protect cerebral ischemia-reperfusion injury by improving the microvascular contraction function
%K 梓葛冻干粉针
%K NOS
%K ET-1
%K TXB&lt
%K sub&gt
%K 2&lt
%K /sub&gt
%K 6-Ke-to-PGF&lt
%K sub&gt
%K 1α&lt
%K /sub&gt
%K 脑微血管舒缩功能&lt
%K br&gt
%K Zige lyophilized powder for injection
%K NOS
%K ET-1
%K TXB&lt
%K sub&gt
%K 2&lt
%K /sub&gt
%K 6-Ke-to-PGF&lt
%K sub&gt
%K 1α&lt
%K /sub&gt
%K microvascular contraction function
%U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2017/6/201706004.htm