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- 2017
基于木瓜蛋白酶过氧化物酶活性检测谷胱甘肽
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Abstract:
木瓜蛋白酶是新发现的具有过氧化物模拟酶活性的蛋白酶，它能催化H2O2氧化3，3'，5，5'-四甲基联苯胺(TMB)，发生蓝色反应，并在652 nm处有吸收峰.当引入谷胱甘肽(GSH)后，由于GSH消耗H2O2，使其在652 nm处吸光度降低，且溶液颜色变浅.基于此，设计了一种方便简单、灵敏的比色分析法检测GSH，在生物样品检测中具有潜在的应用.
Papain, a newly discovered proteinase, possesses peroxidase enzyme activity, which can catalyze the oxidation of the peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the presence of H2O2 to the oxidized TMB to induce a blue colored reaction with a typical absorption peak centered at 652 nm. However, GSH can consume H2O2, which results in a fading of the blue color of the reaction system and a decrease of the absorbance. Accordingly, GSH can be detected by measuring the wastage of H2O2. Based on this approach, a facile, sensitive and selective colorimetric assay has been developed to detect GSH, with a promising application in biological fluids
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