青蒿多糖对小鼠血清中IL-1β，IL-2，IL-6，IFN-γ水平的影响
Effect of Herba Artemisiae Annuae Polysaccharides on IL-1β, IL-2, IL-6 and IFN-γ Levels in the Serum of Mice

为观察青蒿多糖(Herba Artemisiae Annuae polysaccharides，HAAP)对小鼠免疫功能的影响，采用木瓜蛋白酶法提取青蒿多糖，将50只昆明种小鼠随机分成5组进行试验.第Ⅰ组为对照组，腹腔注射生理盐水；第Ⅱ组到第Ⅴ组为青蒿多糖组，分别按25，50，100，150 mg/kg剂量腹腔注射青蒿多糖，连续给药7 d.分离血清测定IL-1β，IL-2，IL-6，IFN-γ质量浓度.结果显示50 mg/kg青蒿多糖能提高血清中IL-1β，IL-2和IFN-γ质量浓度(p＜0.01)；25 mg/kg青蒿多糖也能提高血清中IL-6和IFN-γ质量浓度(p＜0.01)；100 mg/kg青蒿多糖对IL-2有升高作用，对IL-6和IFN-γ有降低作用(p＜0.01).结果表明青蒿多糖对小鼠血清中这4种细胞因子有调节作用.提示适当质量浓度的青蒿多糖可能对Th1/Th2的平衡有调节作用.
In order to observe the effects of HAAP (Herba Artemisiae Annuae polysaccharides) on immune function in mice, HAAP were extracted with papain. Fifty mice were randomly divided into 5 groups. GroupⅠ was the control group, in which the animals were intraperitoneally injected with physiological saline; From Group Ⅱ to GroupⅤ, the animals were intraperitoneally injected with HAAP at 25, 50, 100 and 150 mg/kg, respectively, for 7 successive days. The levels of IL-1β, IL-2, IL-6 and IFN-γ from the separated serum were measured. The results showed that there were no starch, protein or amine acid in HAAP, and the content of total sugar was 24.74%. Compared with the control, HAAP at 50 mg/kg increased the serum levels of IL-1β, IL-2 and IFN-γ (p < 0.01); HAAP at 25 mg/kg increased the serum levels of IL-6 and IFN-γ (p < 0.01); and HAAP at 100 mg/kg increased the level of IL-2 but reduced the levels of IL-6 and IFN-γ (p < 0.01). The above results indicated that HAAP had a regulating effect on the four cytokines in the serum of mice, and suggested that HAAP, at a proper concentration, might probably adjust Th1/Th2 balance