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- 2017
人源类金属硫蛋白能促进番茄幼苗对锌的积累
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Abstract:
金属硫蛋白具有抗辐射、抗氧化和对重金属解毒等功能，对Zn2+具有很强的结合能力，在医药、农业、环境和保健等诸多领域有潜在的应用价值.将花椰菜花叶病毒35S启动子控制的人源类金属硫蛋白基因HsMT1L导入番茄基因组，结果显示，转基因番茄幼苗比野生型材料对Zn2+具有更强的耐受性，并能够富集更多的Zn2+离子.
Metallothionein (MT) has the functions of radio-resistance, oxidation-resistance and heavy-metal detoxification, and has a high binding capacity to Zn2+, and hence it has potential application value in medicine, agriculture, environment protection, health care, and others. In this study, HsMT1L, a metallothionein 1-like gene from human, which is controlled by cauliflower mosaic virus 35S promoter containing duplicated enhancer sequence (2x35S), was introduced into the genome of tomato (Solanum lycopersicum L. cv Ailsa Craig). The results showed that the tomato seedlings containing the 2x35S-HsMT1L exhibited higher Zn2+ tolerance and higher Zn2+ accumulation in the plant than the wild type
| [1] | KIMURA T, KAMBE T. The Functions of Metallothionein and ZIP and ZnT Transporters: An Overview and Perspective[J]. Int J Mol Sci, 2016, 17(3): 336-358. DOI:10.3390/ijms17030336 |
| [2] | 郑军恒, 李海洋, 茹刚, 等. 金属硫蛋白清除羟自由基功能的研究[J]. 北京大学学报(自然科学版), 1999, 35(2): 573-576. |
| [3] | 仪慧兰, 吕品, 吴丽华. 转MT工程菌的构建及其对重金属的抗性研究[J]. 山西大学学报(自然科学版), 2012(2): 395-399. |
| [4] | 陈红亮, 龙黔, 许华杰. 水培条件下不同浓度镉对辣椒的毒害效应[J]. 贵州师范大学学报(自然科学版), 2012, 30(6): 12-15. |
| [5] | 中国科学院南京土壤研究所. 土壤理化分析[M]. 上海: 上海科学技术出版社, 1978: 360-361. |
| [6] | PALMITER R D. The Elusive Fuction of Etallothioneins[J]. Proc Natl Acad Sci USA, 1998, 95: 8428-8430. DOI:10.1073/pnas.95.15.8428 |
| [7] | VALLEE B L. Introduction to Metallothionein[J]. Methods in Enzymology, 1991, 205(4): 3-7. |
| [8] | 常红, 周鑫斌, 于淑慧, 等. 小麦氮锌配施效应研究[J]. 西南大学学报(自然科学版), 2013, 35(11): 49-53. |
| [9] | PALMITER R D, FINDLEY S D, WHITMORE T E, et al. MT-Ⅲ, A Brain-Specific Member of the Metallothionein Gene Family[J]. Proc Natl Acad Sci USA, 1992, 89(14): 6333-6337. DOI:10.1073/pnas.89.14.6333 |
| [10] | 杜小兵, 翟军鹏, 崔萌, 等. 类植物螯合肽的原核表达与Zn2+螯合能力分析[J]. 西南大学学报(自然科学版), 2014, 36(12): 25-29. |
| [11] | MURASHIGE T, SKOOG F. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures[J]. Physiology Plant, 1962, 15(3): 473-497. DOI:10.1111/ppl.1962.15.issue-3 |
| [12] | YANG F, ZHOU M, HE Z, et al. High-Yield Expression in Escherichia coli of Soluble Human MT2A with Native Functions[J]. Protein Expr Purif, 2007, 53(1): 186-194. DOI:10.1016/j.pep.2006.12.007 |
| [13] | SAMBROOK J, FRITSCH E F, MANIATIS T. Molecular Cloning: A Laboratory Manual[M].?: Cold Spring Harbor Laboratory Press, 1989: 237-303. https://www.cabdirect.org/cabdirect/abstract/19901616061 |
| [14] | CHEN H, NELSON R S, SHERWOOD J L. Enhanced Recovery of Transformats of Agrobacterium tumefaciens After Freeze-Thaw Transformation and Drug Selection[J]. Biotechniques, 1994, 16(4): 664-670. |
| [15] | GRAHAM R D, ASCHER J S, HYNES S C. Selecting Zinc-Efficient Cereal Genot Ypes for Soils of Low Zinc Status[J]. Plant and Soil, 1992, 146(1): 241-250. |
| [16] | 生吉萍, 李汉臣, 刘开朗, 等. 转小鼠金属硫蛋白(MT)基因烟草不同器官中金属离子铅和锌的分布[J]. 光谱学与光谱分析杂志, 2008, 28(10): 2401-2403. |
| [17] | 王立芹, 周倜, 徐淑倩, 等. 锌与金属硫蛋白基因表达的研究进展[J]. 中国饲料添加剂, 2014(6): 1-4. |
