%0 Journal Article %T 人源类金属硫蛋白能促进番茄幼苗对锌的积累<br><i>Homo sapiens</i> Metallothionein 1L (HsMT1L) Enhances the Accumulation of Zinc in Tomato Seedlings %A 宋小梅 %A 陈梦莹 %A 秦小冬 %A 金新开 %A 李金华 %A 潘宇 %A 苏承刚 %A 张兴国< %A br> %A SONG Xiao-mei %A CHEN Meng-ying %A QIN Xiao-dong %A JIN Xin-kai %A LI Jin-hua %A PAN Yu %A SU Cheng-gang %A ZHANG Xing-guo %J 西南大学学报(自然科学版) %D 2017 %R 10.13718/j.cnki.xdzk.2017.07.005 %X 金属硫蛋白具有抗辐射、抗氧化和对重金属解毒等功能,对Zn<sup>2+</sup>具有很强的结合能力,在医药、农业、环境和保健等诸多领域有潜在的应用价值.将花椰菜花叶病毒35S启动子控制的人源类金属硫蛋白基因<i>HsMT</i>1<i>L</i>导入番茄基因组,结果显示,转基因番茄幼苗比野生型材料对Zn<sup>2+</sup>具有更强的耐受性,并能够富集更多的Zn<sup>2+</sup>离子.<br>Metallothionein (MT) has the functions of radio-resistance, oxidation-resistance and heavy-metal detoxification, and has a high binding capacity to Zn<sup>2+</sup>, and hence it has potential application value in medicine, agriculture, environment protection, health care, and others. In this study, <i>HsMT</i>1<i>L</i>, a metallothionein 1-like gene from human, which is controlled by cauliflower mosaic virus 35S promoter containing duplicated enhancer sequence (2x35S), was introduced into the genome of tomato (<i>Solanum lycopersicum</i> L. cv Ailsa Craig). The results showed that the tomato seedlings containing the 2x35S-HsMT1L exhibited higher Zn<sup>2+</sup> tolerance and higher Zn<sup>2+</sup> accumulation in the plant than the wild type %K 人源类金属硫蛋白基因 %K 番茄 %K 幼苗 %K 锌< %K br> %K < %K i> %K Homo sapiens< %K /i> %K metallothionein 1L gene (< %K i> %K HsMT< %K /i> %K 1< %K i> %K L< %K /i> %K ) %K < %K i> %K Solanum lycopersicum< %K /i> %K seedling %K zinc %U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2017/7/201707005.htm