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-  2017 

通过硫酯反应检测脂肪酸代谢中间产物 Application of a Novel Thioester Decomposition Reaction in Fatty Acid Biosynthesis Intermediates Analysis

Keywords: 脂肪酸代谢,硫酯反应,高分辨质谱,中间代谢产物

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Abstract:

目的:研究脂肪酸生物合成途径中中间代谢产物的积累情况,并推断其确切结构。方法:利用n-丁胺在牛血清蛋白(BSA)的催化下酯解酰基载体蛋白(ACP)上挂载的脂肪酸中间代谢产物,通过高分辨质谱测定得到的中间代谢产物衍生物的精确分子量,进而获得中间代谢产物的结构信息。结果:建立了一种可以有效截获脂肪酸代谢途径中间代谢物的方法,通过该方法鉴定到了13种不同的中间代谢产物,并推断了这些代谢物的结构。结论:首次确定了脂肪酸中间代谢产物以含有不饱和键的为主,部分中间代谢产物的碳链上含有羟基,导致这两类中间代谢产物的积累可能与烯脂酰ACP还原酶和3-羟基脂酰ACP还原酶的活性不足有关

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