RP-HPLC测定替诺福韦及其有关物质

为了研究替诺福韦的有关物质，合成了3个有关化合物，建立了替诺福韦RP-HPLC分离条件(以Agilent ZORBAX SB-C18为色谱柱，以甲醇-0.02 mol/L磷酸二氢钾为流动相梯度洗脱，检测波长为260 nm)。结果显示替诺福韦有3种潜在的有关物质。所建立的分析方法准确度高，重复性好，适用于替诺福韦有关物质的含量检测。
To investigate the related substances of tenofovir, 3 related substances were synthesized. RP-HPLC analytical method was developed using Agilent ZORBAX SB-C18 column. The mobile phase composed of methanol and 0. 02 mol/L potassium dihydrogen phosphate. Linear gradient elution was used and the detection wavelength was 260 nm. 3 impurities were found in tenofovir. The established method was accurate and reproducible, and it can be applied for the related substances test in tenofovir