微波辅助合成硫酸化发酵灵芝胞外多糖的研究

多糖的硫酸化修饰能提高多糖的生物活性。从灵芝深层发酵液中获取灵芝胞外多糖，以二甲基甲酰胺为反应溶剂，采用氨基磺酸为酯化剂，探讨了微波辅助加热合成多糖的途径。结果表明，将尿素作为催化剂微波辅助合成硫酸酯化多糖，反应条件温和，取代度可高达2.67。因此微波辅助合成法为获取高取代度硫酸化灵芝多糖开辟了一条新途径。
Sulfation of polysaccharides can improve their biological activities. To efficiently prepare the sulfated extracellular polysaccharide from submerged fermentation of Ganoderma Lucidum，microwave assisted synthesis was established in this paper，using dimethylformamide as solvent and aminosulfonic acid as esterifying reagent. Results showed that the microwave assisted synthesis with urea as catalyst led to a high substitution degree of 2.67，and the reaction was mild. Thus，microwave assisted synthesis is a new approach to obtain sulfated polysaccharides with high degree of substitution