基于巨噬细胞模型的竹荪多糖的免疫功能

为探索竹荪多糖（DI）对巨噬细胞RAW264.7免疫功能的影响，分别采用四唑氮蓝（MTT）法、中性红法、Griess法、ELISA法，检测DI对巨噬细胞增殖、吞噬活性、一氧化氮（NO）和白细胞介素1（IL?鄄1）、白细胞介素6（IL?鄄6）、α肿瘤坏死因子（TNF?鄄α）分泌量的影响；运用反转录PCR（RT?鄄PCR）检测iNOS和IL?鄄1、IL?鄄6、TNF?鄄α的mRNA表达量的变化。结果表明，在25~200 μg/mL质量浓度范围内，DI能够明显促进巨噬细胞增殖，增强其吞噬活性。同时，DI能够增强巨噬细胞分泌NO、IL?鄄1、IL?鄄6、TNF?鄄α的能力，且明显提高iNOS、IL?鄄1、IL?鄄6、TNF?鄄α mRNA的表达水平。因此，竹荪多糖对巨噬细胞RAW264.7具有免疫刺激作用。
In order to investigate the immunomodulatory effects of a polysaccharide from Dictyophora indusiata （DI） on the immune function of RAW264.7 cells，the effects of DI on cell proliferation，phagocytosis of RAW264.7 cells，levels of NO，IL-1，IL-6 and TNF-α production were detected by MTT assay，neutral red test，Griess method，and ELISA，respectively. The mRNA expression level of iNOS，IL-1，IL-6 and TNF-α were also measured by reverse transcription polymerase chain reaction （RT-PCR）. The results indicated that DI contributed significantly to the proliferation of the macrophages and the phagocytic activity was at 25~200 μg/mL. In addition，the production of NO，IL-1，IL-6 and TNF-α in macrophages were increased. Further，the expression of iNOS，IL-1，IL-6 and TNF-α mRNA were significantly up-regulated. The DI played immunomodulatory effects on macrophage cell line RAW264.7