Trifolium alexandrinum, an important forage
legume, suffers from narrow genetic base. The present investigation was envisaged to reveal the inter- and intra-species genetic diversity and
lineage among 64 accessions, representing a global collection, of T. alexandrinum;it’s two probable progenitorspecies
(T. salmoneum and T. subterraneum) and thethree
genetically distant species (T. repens, T. vesiculosum, T. michelianum). A set of Simple Sequence Repeats (SSR)
primer-pairs developed from T.
alexandrinum have shown to amplify alleles across the species under study,
suggesting utility of the newly developed resource for assessing molecular diversity among Trifolium species. These SSRs markers together with previously reported SSRs, derived
from T. repens,enabled to reveal high intra-species polymorphism in T. alexandrinum and successfully
discriminate different species investigated in this study. The diverse
accessions determined herein provide a superior resource for further breeding
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