%0 Journal Article
%T Genetic Diversity and Lineage Based on SSR Markers of Two Genomic Resources among <i>Trifolium</i> Collections Held within the Australian Pastures Genebank
%A Devendra R. Malaviya
%A Harsh Raman
%A Brian S. Dear
%A Rosy Raman
%A Ajoy K. Roy
%A Pankaj Kaushal
%A Amaresh Chandra
%A Steve J. Hughes
%J Open Journal of Genetics
%P 1-14
%@ 2162-4461
%D 2019
%I Scientific Research Publishing
%R 10.4236/ojgen.2019.91001
%X <i>Trifolium alexandrinum</span></i><span style=\"font-family:Verdana;\">, an important forage
legume, suffers from narrow genetic base</span><span style=\"font-family:Verdana;\">. T</span><span style=\"font-family:Verdana;\">he present investigation was envisaged to reveal the </span><span style=\"font-family:Verdana;\">inter- and intra-species </span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">genetic diversity and
lineage among 64 accessions, representing a global collection, of </span><i><span style=\"font-family:Verdana;\">T. alexandrinum;</span><span style=\"color:red;\"> </span></i></span><span style=\"font-family:Verdana;\">it¡¯s</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> two probable progenitor</span><i> </i><span style=\"font-family:Verdana;\">species
(</span><i><span style=\"font-family:Verdana;\">T. salmoneum</span></i><span style=\"font-family:Verdana;\"> and </span><i><span style=\"font-family:Verdana;\">T. subterraneum</span></i><span style=\"font-family:Verdana;\">) and the</span><b> </b><span style=\"font-family:Verdana;\">three
genetically distant species (</span><i><span style=\"font-family:Verdana;\">T. repens</span></i><span style=\"font-family:Verdana;\">,</span><i><span style=\"font-family:Verdana;\"> T. vesiculosum</span></i><span style=\"font-family:Verdana;\">,</span><i><span style=\"font-family:Verdana;\"> T. michelianum</span></i><span style=\"font-family:Verdana;\">). A set of Simple Sequence Repeats (SSR)
primer-pairs developed from </span><i><span style=\"font-family:Verdana;\">T.
alexandrinum</span></i><span style=\"font-family:Verdana;\"> have shown to amplify alleles across the species under study,
suggesting utility of the newly developed resource for </span><span style=\"font-family:Verdana;\">assessing molecular diversity among </span><i><span style=\"font-family:Verdana;\">Trifolium</span></i><span style=\"font-family:Verdana;\"> species. These SSRs markers together with previously reported SSRs, derived
from </span><i><span style=\"font-family:Verdana;\">T. repens</span></i><span style=\"font-family:Verdana;\">,</span><i> </i><span style=\"font-family:Verdana;\">enabled to reveal high intra-species polymorphism in </span><i><span style=\"font-family:Verdana;\">T. alexandrinum</span></i><span style=\"font-family:Verdana;\"> and successfully
discriminate different species investigated in this study. The diverse
accessions determined herein provide a </span></span><span style=\"font-family:Verdana;\">superior resource for further breeding</span><span
%K Egyptian Clover
%K Evolution
%K Genetic Resource
%K Molecular Diversity
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=90709