Although animal proteins provide indispensable amino acids that the body requires for normal growth, maintenance and function, their expensiveness makes them unaffordable especially for most families in the developing countries. This has given impetus to extensive research into under-utilized protein-rich oilseeds such as sorrel as possible alternate sources of good quality protein for tackling the challenge of protein-energy malnutrition which is fast becoming a global challenge. Sorrel seed may hold great potentials as a source of good quality protein, however the presence of hard seed coat, bitter after-taste and associated antinutritional factors have limited its use as protein supplement for humans and food ingredient. This study therefore compared the effect of dehulling sorrel seed to boiling, germination and roasting. This was with the aim of enhancing its utilization as protein source for human nutrition and functional ingredient in food product development. Flours obtained were analyzed for their proximate, mineral, antinutrient, amino and fatty acids composition; in vitro starch and protein digestibility, and functional and antioxidative properties. Protein content (ranged from 24.93% - 32.91%) significantly increased due to processing; dehulling alone accounted for a percentage increase of 32.01%. Similarly, dehulling increased all essential amino acids (except isoleucine and valine) at percentage which ranged from 3.63% - 61.17% whereas other processing methods caused significant reductions. Lysine, leucine, valine, arginine and phenylalanine were the most abundant essential amino acids, while methionine and cystine were the first and second limiting amino acids. Palmitic, linoleic, oleic and stearic acids were the most abundant fatty acids. Mineral composition was K > Ca > Mg > Na > Fe > Zn > Mn. Dehulled seed flour had highest in vitro protein digestibility (75.87%). Improved amino acid composition, antioxidative and functional properties of sorrel seed flour due to dehulling may indicate the potential of this flour to serve as a protein supplement and functional ingredient for food product development.
References
[1]
FAO (2005) The State of Food Insecurity in the World 2005. Rome.
http://www.fao.org/3/a-a0200e.pdf
[2]
Müller, O. and Krawinkel, M. (2005) Malnutrition and Health in Developing Countries. CMAJ, 173, 279-286. https://doi.org/10.1503/cmaj.050342
[3]
Duncan, T. (2001) Commission on Macroeconomics and Health. Health, Nutrition and Economic Prosperity: A Microeconomic Perspective. CMH Working Papers No WG1:7. World Health Organization, Geneva.
[4]
Cleaver, K., Okidegbe, N. and De Nys, E. (2006) Agriculture and Rural Development: Hunger and Malnutrition. World Bank Seminar Series: Global Issues Facing Humanity, 1-18.
[5]
Young, V.R. and Pellet, P.L. (1990) Current Concepts Concerning Indispensable Amino Acids Needs in Adults and Their Implications for International Nutrition Planning. Food and Nutrition Bulletin, 12, 289-300.
https://doi.org/10.1177/156482659001200414
[6]
Volgarev, M.N. and Waggle, D.H. (1992) Introduction. In: Steinke, F.H., Waggle, D.H. and Volgarev, M.N., Eds., New Protein Foods in Human Health: Nutrition Prevention and Therapy, CRC Press Inc., Boca Raton and London, 1-8.
[7]
Liener, I.E. (1980) Introduction. In: Liener, I.E., Ed., Toxic Constituents of Plant Food Stuffs, 2nd Edition, Academic Press, Inc., New York, 1-5.
[8]
Bressani, R., Elias, L.G. and Braham, J.E. (1982) Reduction of Digestibility of Legume Protein of Tannins. Journal of Plant Foods, 4, 43-55.
https://doi.org/10.1080/0142968X.1982.11904247
[9]
Wolf, W.J. (1992) Protein Sources for Use in Food Products. In: Steinke, F.H., Waggle, D.H. and Volgarev, M.N., Eds., New Protein Foods In-Human Health: Nutrition, Prevention and Therapy, CRC Press Inc., Boca Raton and London, 33-46.
[10]
Chavan, U., McKenzie, D. and Shahidi, F. (2001) Functional Properties of Protein Isolates from Beach Pea (Lathyrus maritimus L.). Food Chemistry, 74, 177-187.
https://doi.org/10.1016/S0308-8146(01)00123-6
[11]
Al-Wandawi, H., Al-Shaikhly, K. and Abdul-Rahman, M. (1984) Roselle Seed: A New Protein Source. Journal of Agricultural and Food Chemistry, 32, 510-512.
https://doi.org/10.1021/jf00123a022
[12]
Kwari, I.D., Diarra, S.S., Raji, A.O. and Adamu, S.B. (2011) Egg Production and Egg Quality Laying Hens Fed Raw or Processed Sorrel (Hibiscus sabdariffa) Seed Meal. Agriculture and Biology Journal of North America, 2, 616-621.
https://doi.org/10.5251/abjna.2011.2.4.616.621
[13]
Halimatul, S.M.N., Amin, I., Mohd.-Esa, N., Nawalyah, A.G. and SitiMuskinah, M. (2007) Protein Quality of Roselle (Hibiscus sabdariffa L.) Seeds. ASEAN Food Journal, 14, 131-140.
[14]
Amin, I., Hainida, E.K.I. and Halimatul, S.M.N. (2008) Roselle (Hibiscus sabdariffa L.) Seeds-Nutritional Composition, Protein Quality and Health Benefits. Food, 2, 1-16.
[15]
El-Adawy, T.A. and Khalil, A.H. (1994) Characteristic of Roselle Seeds as a New Source of Protein and Lipid. Journal of Agricultural and Food Chemistry, 42, 1896-1900. https://doi.org/10.1021/jf00045a013
[16]
Rao, P.U. (1996) Nutrient Composition and Biological Evaluation of Mesta (Hibiscus sabdariffa) Seeds. Plant Foods in Human Nutrition, 49, 27-34.
https://doi.org/10.1007/BF01092519
[17]
Abu-Tarboush, H.M., Ahmed, S.A.B. and Al Kahtani, H.A. (1997) Some Nutritional and Functional Properties of Karkade (Hibiscus sabdariffa) Seed Products. Cereal Chemistry, 74, 352-355. https://doi.org/10.1094/CCHEM.1997.74.3.352
[18]
Yagoub, A.A. and Mohammed, M.A. (2008) Fururndu, a Meat Substitute from Fermented Roselle (Hibiscus sabdariffa L.) Seed: Investigation on Amino Acids Composition, Protein Fractions, Minerals Content and HCl-Extractability and Microbial Growth. Pakistan Journal of Nutrition, 7, 352-358.
https://doi.org/10.3923/pjn.2008.352.358
[19]
Schippers, R.R. (2000) African Indigenous Vegetable: An Overview of the Cultivated Species. Natural Resources Institute/ACP-EU. Technical Center for Agricultural and Rural Cooperation, Chatam, 1-124.
Cater, C.M., Cravens, W.W., Horan, F.E., Lewis, C.J., Mattil, K.F. and Williams, L.D. (1978) Oilseed Proteins. In: Milner, M., Scrimshaw, N.S. and Wang, D.I.C., Eds., Protein Resources and Technology: Status and Research Needs, 2AVI Publishing Company, West Port, 78-301.
[22]
Oomah, B.D., Mazza, G. and Przybylski, R. (1996) Comparison of Flaxseed Meal Lipids Extracted with Different Solvents. Lebensmittel Wissenschaft und Technologie, 29, 654-658. https://doi.org/10.1006/fstl.1996.0100
[23]
Tasneem, R., Ramsmani, S. and Subramaja, V. (1982) Functional Properties of Guar Seed Cyamopsis tetragonoloba Meal Detoxified by Different Methods. Journal of Food Science, 47, 1323-1328. https://doi.org/10.1111/j.1365-2621.1982.tb07678.x
[24]
Tounkara, F., Amadou, I., Le, G.W. and Shi, Y.H. (2011) Effect of Boiling on the Physicochemical Properties of Roselle Seeds (Hibiscus sabdariffa L.) Cultivated in Mali. African Journal of Biotechnology, 10, 18160-18166.
[25]
Duwa, H., Oyawoye, E.O. and Njidda, A.A. (2012) Effect of Processing Methods on the Utilization of Sorrel Seed Meal by Broilers. Pakistan Journal of Nutrition, 11, 38-46. https://doi.org/10.3923/pjn.2012.38.46
[26]
AOAC (1990) Official Methods of Analysis. Association of Analytical Chemists, Washington DC.
[27]
Isaac, R.C. and Johnson, W.C. (1975) Collaborative Study of Wet and Dry Techniques for the Elemental Analysis of Plant Tissue by Atomic Absorption Spectrophotometer. Journal of the Association of Analytical Chemistry, 58, 436.
[28]
Jones, J. and Case, V. (1990) Sampling, Handling and Analyzing Plant Tissue Samples. In: Westerman, R.L., Ed., Soil Testing and Plant Analysis, SSSA Book Series No. 3, 3rd Edition.
[29]
Oh, K.S. (1994) Changes in Lipid Components of Pollack during Sun Drying. Korean Journal of Food Science and Technology, 18, 123-126.
[30]
Akintayo, E.T. and Bayer, E. (2002) Characterization and Possible Uses of Plukenatia conophora and Adenopus breviflorus Seed and Seed Oil. Bioresource Technology, 85, 95-97. https://doi.org/10.1016/S0960-8524(02)00073-1
[31]
Rodríguez-Amaya, D.B. and Kimura, M. (2004) Harvestplus Handbook for Carotenoid Analysis. International Food Policy Research Institute, Washington DC, 34-36.
[32]
Hsu, H.W., Vavak, D.L., Satterlee, L.D. and Miller, G.A. (1977) A Multi-Enzyme Technique for Estimating Protein Digestibility. Journal of Food Science, 42, 1269-1273. https://doi.org/10.1111/j.1365-2621.1977.tb14476.x
[33]
Sigh, U., Klierdeker, M.S. and Jambunathan, R. (1982) Studies on Desi and Kabu li Chickpeas (Cicer arieinum L.) Cultivars. The Level of Protein Inhibitors, Level of Oligosaccharides and In-Vitro Digestibility. Journal of Food Science, 47, 510-412.
https://doi.org/10.1111/j.1365-2621.1982.tb10113.x
[34]
Butrits, M. and Bucar, F. (2000) Antioxidant Activity of Nigeria Sativa Essential Oil. Phytotheraphy Research, 14, 323-328.
https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q
[35]
Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin Ciocalteu Reagent. Methods Enzymology, 299, 152-177.
https://doi.org/10.1016/S0076-6879(99)99017-1
[36]
Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. (2005) Determination of the Total Phenolic, Flavonoid and Proline Contents in Burkina Fasan Honey, as Well as Their Radical Scavenging Activity. Food Chemistry, 91, 571-577.
https://doi.org/10.1016/j.foodchem.2004.10.006
[37]
Pulido, R., Bravo, L. and Saura-Calixto, F. (2002) Antioxidant Activity of Dietary Polyphenols as Determined by a Modified Ferric Reducing/Antioxidant Power Assay. Journal of Agricultural and Food Chemistry, 48, 3396-3402.
https://doi.org/10.1021/jf9913458
[38]
Rodriguez-Ambriz, S., Martinez-Ayala, A., Millan, F. and Davila-Ortiz, G. (2005) Composition and Functional Properties of Lupinus campestris Protein Isolates. Plant Foods for Human Nutrition, 60, 99-107.
https://doi.org/10.1007/s11130-005-6835-z
[39]
Omowaye-Taiwo, O.A., Fagbemi, T.N., Ogunbusola, E.M. and Badejo, A.A. (2015) Effect of Germination and Fermentation on the Proximate Composition and Functional Properties of Full-Fat and Defatted Cucumeropsis mannii Seed Flours. Journal of Food Science and Technology, 52, 5257-5263.
https://doi.org/10.1007/s13197-014-1569-2
[40]
Ukpabi, U.J. and Ndimele, C. (1990) Evaluation of Quality of Gari Produced in Imo State. Nigerian Food Journal, 8, 106-109.
[41]
Sze-Tao, K.W. and Sathe, S.K. (2000) Functional Properties and in Vitro Digestibility of Almond (Prunus dulcis L.) Protein Isolate. Food Chemistry, 69, 153-160.
https://doi.org/10.1016/S0308-8146(99)00244-7
[42]
Palic, D., Morey, L., Modika, K.Y., Kokic, B., Djuragic, O. and Spasevski, N. (2012) Precision of Laboratory Methods Based on Protein Solubility in Quality Control of Heat Treated Feedstuffs. Chemistry and Industry, 66, 53-57.
https://doi.org/10.2298/HEMIND110623054P
[43]
Wheeler, E.L. and Ferrel, R.E. (1971) A Method for Phytic Acid Determination in Wheat and Wheat Fraction. Journal of Cereal Chemistry, 48, 312-320.
[44]
Makkar, H.P.S. (1994) Quantification of Tannins: A Laboratory Manual. International Centre for Agricultural Research in the Dry Areas (ARDA), Alleppo Syria.
AOAC (2005) Official Methods of Analysis. 18th Editon, Association of Analytical Chemist, Washington DC, 4.
[47]
Emmy Hainida, K.I., Amin, I., Normah, H. and Mohd-Esa, N. (2008) Nutritional and Amino Acid Contents of Differently Treated Roselle (Hibiscus sabdariffa L.) Seeds. Food Chemistry, 111, 906-911.
https://doi.org/10.1016/j.foodchem.2008.04.070
[48]
FAO (2001) Improving Nutrition through Home Gardening-A Training Package for Preparing Field Workers in Africa, Food and Nutrition Division in collaboration with Plant Production and Protection Division, FAO, Rome, Italy.
http://www.fao.org/home/en/
[49]
Yu-Hui, T. (1991) Effect of the Hard-to-Cook Defect and Processing on Protein and Starch Digestibility of Cow-Peas. Cereal Chemistry, 68, 413-418.
[50]
Iyenagbe, D.O., Malomo, S.A., Idowu, A.O., Badejo, A.A. and Fagbemi, T.N. (2017) Effects of Thermal Processing on the Nutritional and Functional Properties of Defatted Conophor Nut (Tetracarpidium conophorum) Flour and Protein Isolates. Food Science and Nutrition, 5, 1170-1178. https://doi.org/10.1002/fsn3.508
[51]
Ogunbusola, E.M., Fagbemi, T.N. and Osundahunsi, O.F. (2013) In-Vitro Protein Digestibility, Amino Acid Profile, Functional Properties and Utilization of White Melon (Cucumeropsis mannii) Protein Isolates. Journal of Food Science and Technology, 4, 153-159.
[52]
Fagbemi, T.N. (2009) Effect of Processing on Chemical Composition of Cashewnut (Anacardium occidentale). Journal of Food Science and Technology, 46, 36-40.
[53]
FAO/WHO (1991) Protein Quality Evaluation. In: Report of Joint FAO/WHO Expert Consultation, Food and Agriculture Organization of the United Nations, Rome, FAO Food and Nutrition Paper 51.
[54]
FAO/WHO (2007) Protein Quality Evaluation. In: Report of the Joint FAO/WHO Expert Consultation, Food and Agriculture Organizations and the World Health Organization, Rome, Food and Nutrition No. 69.
[55]
Malomo, S.A., He, R. and Aluko, R.E. (2014) Structural and Functional Properties of Hemp Seed Protein Products. Journal of Food Science, 79, C1512-C1521.
https://doi.org/10.1111/1750-3841.12537
[56]
Mohamed, R., Fernandez, J., Pineda, M. and Aguilar, M. (2007) Roselle (Hibiscus sabdariffa) Seed Oil Is a Rich Source of Gamma-Tocopherol. Journal of Food Science, 72, 207-211. https://doi.org/10.1111/j.1750-3841.2007.00285.x
[57]
Fang, Y.Z., Yang, S. and Wu, G. (2002) Free Radicals, Antioxidants, and Nutrition. Nutrition, 18, 872-879. https://doi.org/10.1016/S0899-9007(02)00916-4
[58]
Baenas, N., Garcia-Viguera, C. and Moreno, D.A. (2014) Elicitation: A Tool for Enriching the Bioactive Composition of Foods. Molecules, 19, 13541-13563.
https://doi.org/10.3390/molecules190913541
[59]
Yamaguchi, T., Takamura, H., Matoba, T. and Terao, J. (1998) HPLC Method for Evaluation of the Free Radical-Scavenging Activity of Foods by Using 1,1-diphenyl-2-picrylhydrazyl. Bioscience, Biotechnology, and Biochemistry, 62, 1201-1204.
https://doi.org/10.1271/bbb.62.1201
[60]
Altschul, A.M. and Wilcke, H.L. (1985) New Protein Foods: Food Science and Technology, a Series of Mongraphs.
[61]
Prinyawiwutku, W., McWatters, K.H., Beuchat, L.R. and Phillips, R.D. (1994) Physical Properties of Cowpea Paste and Akara as Affected by Supplementation with Peanut Flour. Food Chemistry, 42, 1750-1756. https://doi.org/10.1021/jf00044a034
[62]
Oshodi, A.A. and Ekperijin, N.M. (1989) Functional Properties of Pigeon Pea Flour (Cajanus cajan). Food Chemistry, 34, 187-191.
https://doi.org/10.1016/0308-8146(89)90139-8
[63]
Akinyede, A.I., Amoo, I.A. and Eleyinmi, A.F. (2005) Chemical and Functional Properties of Full Fat and Defatted Dioclea reflexa Seed Flours. Journal of Food, Agriculture and Environment, 3, 112-115. http://www.world-food.net
[64]
Fagbemi, T.N., Oshodi, A.A. and Ipinmoroti, K.O. (2006) Effects of Processing on Functional Properties of Full Fat and Defatted Fluted Pumpkin (Telfaria accidentalis) Seed Flours. Journal of Food Technology, 4, 70-79.
[65]
Olaofe, O., Adeyemi, F.O. and Adediran, G.O. (1994) Amino Acid, Chemical Composition and Functional Properties of Some Oil Seeds. Journal of Agricultural and Food Chemistry, 42, 879-881. https://doi.org/10.1021/jf00040a007
[66]
Zheng, G.H. and Sosulski, F.W. (1997) Physicochemical Properties of Small Granule Starches. AACC Annual Meeting, San Diego.
[67]
Lin, M.J.Y., Humbert, E.S. and Sosulski, F.W. (1974) Certain Functional Properties of Sunflower Meal Products. Journal of Food Science, 39, 368-370.
https://doi.org/10.1111/j.1365-2621.1974.tb02896.x
[68]
Mcwatters, K.H. and Cherry, J.P. (1981) Emulsification Vegetable Proteinsin “Protein Functionality in Foods”. American Chemical Society, Washington DC, 217-242. https://doi.org/10.1021/bk-1981-0147.ch011