Environmental dissemination of antimicrobial
resistance genes may occur through agricultural residues, such as animal
manure. We studied the resistome of 16 pool samples of animal manure (pig
slurry [n = 8] and poultry manure [n = 8]), and 16 soil samples (manure-amended
[n = 8] and nonmanure-amended [n = 8]). All samples were collected in central
Spain. Detection was based on 18 selected antimicrobial resistance genes
(ARGs). The most commonly detected genes in animal manure were sul1(16/16), sul2 (16/16), tet(A)
(16/16), aadA (16/16), tet(B)
(15/16), and str (15/16). Genes blaTEM (7/8), mecA (6/8), vanA (5/8) and qnrB (4/8)
were more frequently detected in chicken manure, whereas pig slurry samples
presented higher levels of tet(C) (8/8) and tet(M) (8/8). Out of the
four genes selected for their clinical relevance, three—blaCTX-M
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