Cubiu fruits (Solanum sessiliflorum Dunal, Solanaceae) are known for their high nutritional
value and low caloric content. This work aimed at evaluating biochemical
indices of cubiu fruits according to different tissues (peel, pulp and placenta) and ripening
stages (green, turning, ripe and fully ripe). The fruits were randomly harvested
to investigate sensory aspects (colorimetry, blanching effect, pigments) and
biochemical indices (moisture and dry matter, Total Soluble Solids (TSS), pH, Titratable
Acidity (TA), TSS/TA ratio, ascorbic acid, Alcohol-Insoluble Solids (AIS),
pectinesterase activity and pectin content). The analyses were performed at the laboratory
of Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, Brazil.
The blanching process preserved pulp colors at all ripening stages. Chlorophylls were
higher in green peels; flavonoids and carotenoids, in fully ripe peels. Anthocyanins
were entirely absent. Pulp (turning fruits) showed the highest moisture content
(91.05), followed by ripe pulp (90.70) and fully ripe pulp (90.62). Pulp TSS changed
little whereas placenta TSS declined and were associated with increased TA and pH
reduction (fully ripe fruits). Pulp pH and TA varied little whereas placenta pH was
notably low and TA was remarkably high (fully ripe fruits). Pulp TSS/TA ratio
showed predominance of TSS while in placenta, there was predominance of organic
acids. AIS, precursors of pectin, were stable during ripening. The statistical analysis
of dietary fiber content showed one modal value in the AIS. In pulp, pectinesterase
activity correlated inversely with pectin content. Cubiu fruits were significant sources
of bioactive compounds, e.g. chlorophylls, flavonoids and carotenoids, predominantly
in the peel and the pulp; soluble functional fibers, e.g. pectin (g/100 g fresh weight), particularly in the green peel (1.00) and the fully ripe pulp (1.12), and other
versatile molecules, e.g. ascorbic acid (mg/100 g fresh weight), especially in the fully
ripe peel (32.45) and placenta (24.84) and the turning placenta (21.27). Cubiu fruits
are rich in ascorbic acid and should be included in the human diet.
References
[1]
Andrade Jr., M.C. and Andrade, J.S. (2012) Physicochemical Changes in Cubiu Fruits (Solanum sessiliflorum Dunal) at Different Ripening Stages. Food Science and Technology, 32, 250-254. http://dx.doi.org/10.1590/S0101-20612012005000049
[2]
Whalen, M., Costish, D. and Heiser, C. (1981) Taxonomy of Solanum Section Lasiocarpa. Gentes Herbarum, 12, 41-129.
[3]
Andrade, J.S., Freitas, F.M.N.O., Caceres, L.G. and Figueiredo, J.N.R. (2013) Technologies for Drying Cubiu (Solanum sessiliflorum Dunal) [Tecnologias para Secagem do Cubiu (Solanum sessiliflorum Dunal)]. In: Noda, H., Souza, L.A.G. and Silva Filho, D.F., Eds., Family Agriculture in Amazonas: Conservation of Environmental Resources [Agricultura Familiar No Amazonas: Conservacao dos Recursos Ambientais], Wega, Manaus, 57-70.
[4]
Silva, D.F.P., Rocha, R.H.C. and Salomao, L.C.C. (2011) Postharvest Quality of Cocona (Solanum sessiliflorum Dunal) Stored under Ambient Condition. Revista Ceres, 58, 476-480. http://dx.doi.org/10.1590/s0034-737x2011000400011
Andrade Jr., M.C. and Andrade, J.S. (2014) Amazonian Fruits: An Overview of Nutrients, Calories and Use in Metabolic Disorders. Food and Nutrition Sciences, 5, 1692-1703. http://www.scirp.org/journal/PaperInformation.aspx?PaperID=49580&#abstract http://dx.doi.org/10.4236/fns.2014.517182
[7]
Berry, T. and Bewley, J.D. (1992) A Role for the Surrounding Fruit Tissues in Preventing the Germination of Tomato (Lycopersicon esculentum) Seeds. Plant Physiology, 100, 951-957. http://dx.doi.org/10.1104/pp.100.2.951
[8]
Yuyama, L.K.O., Pantoja, L., Maeda, R.N., Aguiar, J.P.L. and Silva S.B.D. (2008) Development and Acceptability of a Low Calorie Cubiu (Solanum sessiliflorum Dunal) Jam. Food Science and Technology, 28, 929-934. http://dx.doi.org/10.1590/S0101-20612008000400026
[9]
Alexander, L. and Grierson, D. (2002) Ethylene Biosynthesis and Action in Tomato: A Model for Climacteric Fruit Ripening. Journal of Experimental Botany, 53, 2039-2055. http://dx.doi.org/10.1093/jxb/erf072
[10]
Infante, R., Rubio, P., Contador, L., Noferini, M. and Costa, F. (2011) Ripe Nectarines Segregated through Sensory Quality Evaluation and Electronic Nose Assessment. Fruits, 66, 109-119. http://dx.doi.org/10.1051/fruits/2011020
[11]
Infante, R., Rubio, P., Contador, L., Noferini, M. and Costa, F. (2011) Determination of Harvest Maturity of D’Agen Plums Using the Chlorophyll Absorbance Index. Ciencia e Investigación Agraria, 38, 199-203. http://dx.doi.org/10.4067/S0718-16202011000200004
[12]
Wills, R.B.H., Lee, T.H., Graham, D., McGlasson, W.B. and Hall, E.G. (1982) Postharvest: An Introduction to the Physiology and Handling of Fruit and Vegetables. New South Wales University Press Limited, Kensington.
[13]
Wang, Y. and Chen, R. (2014) Regulation of Compound Leaf Development. Plants, 3, 1-17. http://dx.doi.org/10.3390/plants3010001
[14]
Féret, J.-B., Francois, C., Gitelson, A., Asner, G.P., Barry, K.M., Panigada, C., Richardson, A.D. and Jacquemoud, S. (2011) Optimizing Spectral Indices and Chemometric Analysis of Leaf Chemical Properties Using Radiative Transfer Modeling. Remote Sensing of Environment, 115, 2742-2750. http://dx.doi.org/10.1016/j.rse.2011.06.016
[15]
Ashan, G. and Pfanz, H. (2003) Non-Foliar Photosynthesis—A Strategy of Additional Carbon Acquisition. Flora—Morphology, Distribution, Functional Ecology of Plants, 198, 81-97. http://dx.doi.org/10.1078/0367-2530-00080
[16]
Martinelli, F., Basile, B., Morelli, G., D’Andria, R. and Tonutti, P. (2012) Effects of Irrigation on Fruit Ripening Behavior and Metabolic Changes in Olive. Scientia Horticulturae, 144, 201-207. http://dx.doi.org/10.1016/j.scienta.2012.07.012
[17]
Andrade Jr., M.C. and Andrade, J.S. (2015) Changes in Pectinases, Dietary Fibers, and Physicochemical Indices Related to the Flavor of Cubiu Fruits During Ripening. Acta Scientiarum-Agronomy, 37, 171-179. http://dx.doi.org/10.4025/actasciagron.v37i2.19683
[18]
Lichanporn, I., Srilaong, V., Wongs-Aree, C. and Kanlayanarat, S. (2009) Postharvest Physiology and Browning of Longkong (Aglaia dookkoo Griff.) Fruit under Ambient Conditions. Postharvest Biology and Technology, 52, 294-299. http://dx.doi.org/10.1016/j.postharvbio.2009.01.003
[19]
Landi, M., Fambrini, M., Basile, A., Salvini, M., Guidi, L. and Pugliesi, C. (2015) Overexpression of L-Galactono-1,4-Lactone Dehydrogenase (L-GalLDH) Gene Correlates with Increased Ascorbate Concentration and Reduced Browning in Leaves of Lactuca sativa L. after Cutting. Plant Cell, Tissue and Organ Culture, 123, 109-120. http://dx.doi.org/10.1007/s11240-015-0819-y
[20]
Villamiel, M., Del Castillo, M.D. and Corzo, N. (2006) Browning Reactions. In: Hui, Y.H., Ed., Food Biochemistry and Food Processing, Blackwell Publishing, Ames, 71-100. http://dx.doi.org/10.1002/9780470277577.ch4
[21]
Joy, O. and Eze, S.O.O. (2015) Partial Purification and Characterizaton of Peroxidase Extracted from Gongronema latifolium. American-Eurasian Journal of Scientific Research, 10, 221-227.
[22]
Jittanit, W., Wiriyaputtipong, S., Charoenpornworanam, H. and Songsermpong, S. (2011) Effects of Varieties, Heat Pretreatment and UHT Conditions on the Sugarcane Juice Quality. Chiang Mai Journal of Science, 38, 116-125.
[23]
Wiegand, E.H., Madsen, H.S. and Price, F.E. (1943) Commercial Dehydration of Fruits and Vegetables. Oregon Agricultural Experiment Station Bulletin, 417, 3-39.
[24]
Spallholz, J.E., Boylan, L.M. and Driskell, J.A. (1999) Nutrition: Chemistry and Biology. CRC Press, Boca Raton.
[25]
Inanc, A.L. (2011) Chlorophyll: Structural Properties, Health Benefits and Its Occurrence in Virgin Olive Oils. Akademik Gida, 9, 26-32.
[26]
Song, N., Yang, H., Pang, W., Qie, Z., Lu, H., Tan, L., Li, H., Sun, S., Lian, F., Qin, C. and Jiang, Y. (2014) Mulberry Extracts Alleviate a β25-β35-Induced Injury and Change the Gene Expression Profile in PC12 Cells. Evidence-Based Complementary and Alternative Medicine, 2014, Article ID: 150617. http://dx.doi.org/10.1155/2014/150617
[27]
Souza, M.P., Vaz, A.F.M., Cerqueira, M.A., Texeira, J.A., Vicente, A.A. and Carneiro-da-Cunha, M.G. (2015) Effect of an Edible Nanomultilayer Coating by Electrostatic Self-Assembly on the Shelf Life of Fresh-Cut Mangoes. Food Bioprocess Technology, 8, 647-654. http://dx.doi.org/10.1007/s11947-014-1436-1
[28]
Magwazaa, L.S. and Oparab, U.L. (2015) Analytical Methods for Determination of Sugars and Sweetness of Horticultural Products—A Review. Scientia Horticulturae, 184, 179-192. http://dx.doi.org/10.1016/j.scienta.2015.01.001
[29]
Armesto, J., Carballo, J. and Martínez, S. (2015) Physicochemical and Phytochemical Properties of Two Phenotypes of Galega Kale (Brassica oleracea L. var. Acephala cv. Galega). Journal of Food Biochemistry, 39, 439-448. http://dx.doi.org/10.1111/jfbc.12151
[30]
Keng, S.E., Al-Karkhi, A.F.M., Talib, M.K.M., Easa, A.M. and Hoong, C.L. (2015) An Investigation of Potential Fraud in Commercial Orange Juice Products in Malaysian Market by Cluster Analysis and Principal Component Analysis. Malaysian Journal of Analytical Sciences, 19, 377-387.
[31]
Dorofejeva, K., Rakcejeva, T., Galoburda, R., Dukalska, L. and Kviesis, J. (2011) Vitamin C Content in Latvian Cranberries Dried in Convective and Microwave Vacuum Driers. Procedia Food Science, 1, 433-440. http://dx.doi.org/10.1016/j.profoo.2011.09.067
[32]
Alvarez, R., Carvalho, C.P., Sierra, J., Lara, O., Cardona, D. and Londono-Londono, J. (2012) Citrus Juice Extraction Systems: Effect on Chemical Composition and Antioxidant Activity of Clementine Juice. Journal of Agricultural and Food Chemistry, 60, 774-781. http://dx.doi.org/10.1021/jf203353h
[33]
Voragen, A.G.J., Coenen, G.-J., Verhoef, R.P. and Schols, H.A. (2009) Pectin, a Versatile Polysaccharide Present in Plant Cell Walls. Structural Chemistry, 20, 263-275. http://dx.doi.org/10.1007/s11224-009-9442-z
[34]
Liu, Y., Che, F., Wang, L., Meng, R., Zhang, X. and Zhao, Z. (2013) Fruit Coloration and Anthocyanin Biosynthesis after Bag Removal in Non-Red and Red Apples (Malus × domestica Borkh.). Molecules, 18, 1549-1563. http://dx.doi.org/10.3390/molecules18021549
[35]
McGuire, R.G. (1992) Reporting of Objective Color Measurements. HortScience, 27, 1254-1255.
[36]
Gozlekci, S., Saracoglu, O., Onursal, E. and Ozgen, M. (2011) Total Phenolic Distribution of Juice, Peel, and Seed Extracts of Four Pomegranate Cultivars. Pharmacognosy Magazine, 7, 161-164. http://dx.doi.org/10.4103/0973-1296.80681
[37]
HUNTERLAB (1996) CIE L*a*b* Color Scale. Application Note, 8, 1-4.
[38]
Yap, A.U.J., Tan, K.B.C. and Sameer, B. (1997) Comparison of Aesthetic Properties of Tooth-Colored Restorative Materials. Operative Dentistry, 22, 167-172.
[39]
Oke, M. and Paliyath, G. (2006) Biochemistry of Vegetable Processing. In: Hui, Y.H., Ed., Food Biochemistry and Food Processing, Blackwell Publishing, Ames, 537-554. http://dx.doi.org/10.1002/9780470277577.ch23
[40]
Potter, N.N. and Hotchkiss, J.H. (1998) Food Science. An Aspen Publication, Gaithersburg.
[41]
Jen, J.J. and Robinson, M.L. (1984) Pectolytic Enzymes in Sweet Bell Peppers (Capsicum annum L.). Journal of Food Science, 49, 1085-1087. http://dx.doi.org/10.1111/j.1365-2621.1984.tb10398.x
[42]
Andrade, J.S. (1991) Maturation Curves and Nutritional Characteristics of Camu-Camu Myrciaria duabia (HBK) Mark Vaughn Grown in Upland in the Brazilian Central Amazonia [Curvas de Maturacao e Características Nutricionais do Camu-Camu Myrciaria duabia (HBK). Mc Vaugh Cultivado em Terra Firme na Amazonia Central Brasileira]. Thesis, Universidade Estadual de Campinas (UNICAMP), Campinas.
[43]
Xie, X., Gu, W., Gao, S., Lu, S., Li, J., Pan, G., Wang, G. and Shen, S. (2013) Alternative Electron Transports Participate in the Maintenance of Violaxanthin De-Epoxidase Activity of Ulva sp. under Low Irradiance. PLoS ONE, 8, e78211. http://dx.doi.org/10.1371/journal.pone.0078211
[44]
Arnon, D.I. (1949) Copper Enzymes in Isolated Chloroplasts. Polyphenoloxidase in Beta vulagaris. Plant Physiology, 24, 1-15. http://dx.doi.org/10.1104/pp.24.1.1
[45]
Passos, L.P. (1996) Analytical and Laboratory Methods in Plant Physiology, Métodos Analíticos e Laboratoriais em Fisiologia Vegetal. EMBRAPA-CNPGL, Coronel Pacheco.
[46]
Trindade Jr., G., Argolo, R.C., Da Silva, M.V., Teixeira, H.L. and Rego Júnior, N.O. (2010) Irrigation Effect on the Condensed Tannin Levels in Tanzania Grass [Efeito da Irrigacao sobre os Teores de Taninos Condensados em Capim Tanzania]. Publicacoes em Medicina Veterinária e Zootecnia, 4, 1-12.
[47]
Lees, D.H. and Francis, F.J. (1972) Standardization of Pigment Analyses in Cranberries. HortScience, 7, 83-84.
[48]
De Albuquerque, J.R.T., Dos Santos Formiga, A., Da Rocha, T.C., Da Costa, F.B. and De Oliveira Gondim, A.R. (2015) Beet Postharvest Quality Subjected to Fertilization with Fermented Biofertilizers [Qualidade Pós-colheita de Beterraba Submetida à Adubacao com Biofertilizante Fermentado]. Revista Verde de Agroecologia e Desenvolvimento Sustentável, 10, 41-46. http://dx.doi.org/10.18378/rvads.v10i3.3652
[49]
Xiao, H.-W., Bai, J.-W., Sun, D.-W. and Gao, Z.-J. (2014) The Application of Superheated Steam Impingement Blanching (SSIB) in Agricultural Products Processing—A Review. Journal of Food Engineering, 132, 39-47. http://dx.doi.org/10.1016/j.jfoodeng.2014.01.032
[50]
Elfnesh, F., Tekalign, T. and Solomon, W. (2011) Processing Quality of Improved Potato (Solanum tuberosum L.) Cultivars as Influenced by Growing Environment and Blanching. African Journal of Food Science, 5, 324-332.
[51]
Badwaik, L.S., Gautam, G. and Deka S.C. (2015) Influence of Blanching on Antioxidant, Nutritional and Physical Properties of Bamboo Shoot. Journal of Agricultural Sciences, 10, 140-150. http://dx.doi.org/10.4038/jas.v10i3.8067
[52]
Chaimanee, P. and Suntornwat, O. (1994) Changes in Carbohydrate Content During Fruit Ripening—A New Approach of Teaching of Carbohydrate Chemistry in Biochemistry Course. Biochemical Education, 22, 101-102. http://dx.doi.org/10.1016/0307-4412(94)90098-1
[53]
Rosales-Reynoso, O.L., Agama-Acevedo, E., Aguirre-Cruz, A., Bello-Perez, L.A., Dufour, D. and Gibert, O. (2014) Physicochemical Evaluation of Cooking and Dessert Bananas (Musa sp.) Varieties. Agrociencia, 48, 387-401.
[54]
Fuentesa, C.A., Tingb, K.W., Dupont-Gillainc, C., Steensmad, M., Talmad, A.G., Zuijderduind, R. and Van Vuurea, A.W. (2016) Effect of Humidity during Manufacturing on the Interfacial Strength of Non-Pre-Dried Flax Fibre/Unsaturated Polyester Composites. Composites Part A: Applied Science and Manufacturing, 84, 209-215. http://dx.doi.org/10.1016/j.compositesa.2016.01.023
[55]
Masuda, Y. (1968) Role of Cell-Wall-Degrading Enzymes in Cell-Wall Loosening in Oat Coleoptiles. Planta, 83, 171-184. http://dx.doi.org/10.1007/BF00385022
[56]
Andrade Jr., M.C. and Andrade, J.S. (2015) Fermented Foods in General and Ethnic Fermented Foods in Particular. LAP Lambert Academic Publishing, Saarbrüken.
[57]
Eswaran, S., Muir, J. and Chey, W.D. (2013) Fiber and Functional Gastrointestinal Disorders. The American Journal of Gastroenterology, 108, 718-727. http://dx.doi.org/10.1038/ajg.2013.63
[58]
Bresinsky, A., Korner, C., Kadereit, J.W., Neuhaus, G. and Sonnewald, U. (2012) Strasburger’s Textbook of Botany [Tratado de Botanica de Strasburger]. ARTMED, Porto Alegre.
[59]
Bompadre, M.J., Fernández Bidondo, L., Silvani, V.A., Colombo, R.P., Pérgola, M., Pardo, A.G. and Godeas, A.M. (2015) Combined Effects of Arbuscular Mycorrhizal Fungi and Exogenous Cytokinins on Pomegranate (Punica granatum) under Two Contrasting Water Availability Conditions. Symbiosis, 65, 55-63. http://dx.doi.org/10.1007/s13199-015-0318-2
[60]
Yuan, J.-M., Gao, Y.-T., Ong, C.-N., Ross, R.K. and Yu, M.C. (2006) Prediagnostic Level of Serum Retinol in Relation to Reduced Risk of Hepatocellular Carcinoma. Journal of the National Cancer Institute, 98, 482-490. http://dx.doi.org/10.1093/jnci/djj104
[61]
Tang, G. (2001) Bioconversion of Dietary Provitamin A Carotenoids to Vitamin A in Humans. American Journal of Clinical Nutrition, 91, 1468S-1473S. http://dx.doi.org/10.3945/ajcn.2010.28674G
[62]
Harrison, E.H. (2012) Mechanisms Involved in the Intestinal Absorption of Dietary Vitamin A and Provitamin A Carotenoids. Biochimica et Biophysica Acta, 1821, 70-77. http://dx.doi.org/10.1016/j.bbalip.2011.06.002
[63]
Plaisancié, J., Brémond-Gignac, D., Demeer, B., Gaston, V., Verloes, A., Fares-Taie, L., Gerber, S., Rozet, J.-M., Calvas, P. and Chassaing, N. (2016) Incomplete Penetrance of Biallelic ALDH1A3 Mutations. European Journal of Medical Genetics, 59, 215-218. http://dx.doi.org/10.1016/j.ejmg.2016.02.004
[64]
National Academy of Sciences (2000) Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. National Academy Press, Washington DC.
[65]
Schaneberg, B. (2011) AOAC Proposal on Flavonoid Measurement and Terminology. AOAC International, Rockville.
[66]
Mandel, T., Rogachev, I., Venger, I., Mintz-Oron, S., Aharoni, A. and Adato A. (2007) The Ins and Outs of Tomato Fruit Peel Metabolome. Acta Horticulturae, 814, 465-474.
[67]
Lara, I., Belge, B. and Goulao, L.F. (2015) A Focus on the Biosynthesis and Composition of Cuticle in Fruits. Journal of Agricultural and Food Chemistry, 63, 4005-4019. http://dx.doi.org/10.1021/acs.jafc.5b00013
[68]
Lownds, N.K., Banaras, M. and Bosland, P.W. (1994) Postharvest Water Loss and Storage Quality of Nine Pepper (Capsicum) Cultivars. HortScience, 29, 191-193.
[69]
Díaz-Pérez, J.C., Muy-Rangel, M.D. and Mascorro, A.G. (2007) Fruit Size and Stage of Ripeness Affect Postharvest Water Loss in Bell Pepper Fruit (Capsicum annuum L.). Journal of the Science of Food and Agriculture, 87, 68-73. http://dx.doi.org/10.1002/jsfa.2672
[70]
Tapia, M.R., Gutierrez-Pacheco, M.M., Vazquez-Armenta, F.J., Aguilar, G.A.G., Zavala, J.F.A., Rahman, M.S. and Siddiqui, M.W. (2015) Washing, Peeling and Cutting of Fresh- Cut Fruits and Vegetables. In: Siddiqui, M.W. and Rahman, M.S., Eds., Minimally Processed Foods, Springer, Berlin, 57-78. http://dx.doi.org/10.1007/978-3-319-10677-9_4
[71]
Cunha, A.G. (1991) Nova Fronteira Etymological Dictionary of the Portuguese Language [Dicionário Etimológico Nova Fronteira da Língua Portuguesa]. Nova Fronteira, Rio de Janeiro.
[72]
Hasegawa, S. and Miyake, M. (1996) Biochemistry and Biological Functions of Citrus Limonoids. Food Reviews International, 12, 413-435. http://dx.doi.org/10.1080/87559129609541089
[73]
Correia, S., Goncalves, B., Aires, A., Silva, A., Ferreira, L., Carvalho, R., Fernandes, H., Freitas, C., Carnide, V. and Silva, A.P. (2016) Effect of Harvest Year and Altitude on Nutritional and Biometric Characteristics of Blueberry Cultivars. Journal of Chemistry, 2016, Article ID: 8648609. http://dx.doi.org/10.1155/2016/8648609
[74]
Magiorkinis, E., Beloukas, A. and Diamantis, A. (2011) Scurvy: Past, Present and Future. European Journal of Internal Medicine, 22, 147-152. http://dx.doi.org/10.1016/j.ejim.2010.10.006
[75]
Trippe, B.S., Barrentine, L.W., Curole, M.V. and Tipa, E. (2016) Nutritional Management of Patients with Diabetic Peripheral Neuropathy with L-Methylfolate-Methylcobalamin-Pyridoxal-5-Phosphate: Results of a Real-World Patient Experience Trial. Current Medical Research & Opinion, 32, 219-227. http://dx.doi.org/10.1185/03007995.2015.1103215
[76]
Siegel, B.V. (1993) Vitamin C and the Immune Response in Health and Disease. In: Klurfeld, D.M., Ed., Nutrition and Immunology, Springer, New York, 167-196. http://dx.doi.org/10.1007/978-1-4615-2900-2_8
[77]
Moser, M.A. and Chun, O.K. (2016) Vitamin C and Heart Health: A Review Based on Findings from Epidemiologic Studies. International Journal of Molecular Sciences, 17, 1328. http://dx.doi.org/10.3390/ijms17081328
[78]
Unlu, A., Kirca, O., Ozdogan, M. and Nayir, E. (2016) High-Dose Vitamin C and Cancer. Journal of Oncological Science, 1, 10-12. http://dx.doi.org/10.1016/j.jons.2015.11.010
[79]
Van der Reest, J. and Gottlieb, E. (2016) Anti-Cancer Effects of Vitamin C Revisited. Cell Research, 26, 269-270. http://dx.doi.org/10.1038/cr.2016.7
[80]
Ayala-Zavala, J.F., Vega-Vega, V., Rosas-Domínguez, C., Palafox-Carlos, H., Villa-Rodriguez, J.A., Siddiqui, M.W., Dávila-Avina, J.E. and González-Aguilar, G.A. (2011) Agro -Industrial Potential of Exotic Fruit Byproducts as a Source of Food Additives. Food Research International, 44, 1866-1874. http://dx.doi.org/10.1016/j.foodres.2011.02.021
[81]
Cayupán, Y.S.C., Ochoa, M.J. and Nazareno, M.A. (2011) Health-Promoting Substances and Antioxidant Properties of Opuntia sp. Fruits. Changes in Bioactive-Compound Contents during Ripening Process. Food Chemistry, 126, 514-519. http://dx.doi.org/10.1016/j.foodchem.2010.11.033
