Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of Brucea sumatrana (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo
Results from the in
vitro evaluation of the antiparasitaire activity of the aqueous extract,
the 80% methanol extract and its fractions from the leaves of Brucea sumatrana against Trypanosoma brucei brucei, T. cruzi, Leishmania infantum, the multidrug-resistant K1 and
chloroquine-sensitive NF54 strains of Plasmodium
falciparum indicated that all samples from the leaves extract presented
interesting antiparasitaire activity at different extents. The 80% methanol
extract, its chloroform acid, petroleum ether and 80% methanol soluble
fractions and the aqueous extract exhibited strong activity against Trypanosoma b. brucei, T. cruzi, L. infantum and the multidrug-resistant K1 strain of P. falciparum with IC50 values from <0.25 to 4.35 μg/ml as well as against chloroquine-sensitive
NF54 strain of P. falciparum with IC50 values ranging from <0.02 to 2.0.4 μg/ml. Most samples were cytotoxic
against MRC-5 cell lines (0.2 < cytotoxic concentration 50(CC50)
< 34.24 μg/ml) and showed good selective effect against all tested
parasites. In acute toxicity, the aqueous extract was found to be non-toxic and
its LD50 was estimated to be greater than 5
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