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吉林农业大学学报 2015
rp-hplc法测定鲜人参、生晒参、红参中6种低分子量有机酸Keywords: rp-hplc,freshginseng,whiteginseng,redginseng,lowmolecularweightorganicacid Abstract: ？sixlowmolecularweightorganicacids(aceticacid,malonicacid,lacticacid,malicacid,citricacidandsuccinicacidin)infreshginseng,whiteginsengandredginsengweredeterminedbyreversedphasehighperformanceliquidchromatography(rp-hplc).kromasilc18column（250mm×4.6mm,5μm）wasusedat30℃andmobilephasewas5%ch3oh0.01mol/lk2hpo4(ph2.5)withflowrateof1ml/minandinjectionvolumewas20μl.detectionwavelengthwas210nm.sixorganicacidshadagoodlinearityinrangeof0.001？6-1.0000mg/ml,andaveragerecoveriesofthesixorganicacidswereintherangeof97.54%to102.53%.thismethodhasaccuratelyandeffectivelydeterminedlowmolecularweightorganicacidsinpanaxginseng.
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