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OALib Journal期刊
ISSN: 2333-9721
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不对称还原丙酮酸乙酯菌株的筛选及鉴定
DOI: 10.3724/SP.J.1145.2012.00460, PP. 460-464
Keywords: 丙酮酸乙酯,不对称还原,(s)-乳酸乙酯,静息细胞,26srdnad1/d2,胶红酵母
Abstract:
以丙酮酸乙酯为底物，从成都某化工厂污水池及其附近土壤中分离到36株可将丙酮酸乙酯不对称还原成(s)-乳酸乙酯的菌株.经过多次复筛，最终获得了一株具有较高催化活性的酵母菌bty18-6.在以该菌株静息细胞为催化剂，催化不对称还原丙酮酸乙酯合成(s)-乳酸乙酯的反应中，底物浓度为60mmol/l时，底物转化率为86.9%，产物(s)-乳酸乙酯的ee值为88.7%.通过对其形态学、生理生化特征及其26srdnadl/d2区域的分析表明，bty18-6为胶红酵母.
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