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OALib Journal期刊
ISSN: 2333-9721
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固定化枯草芽孢杆菌发酵生产捷安肽素
DOI: 10.3724/SP.J.1145.2009.00230, PP. 230-234
Keywords: 固定化细胞,捷安肽素,枯草芽孢杆菌,木屑,粘稠剂,响应面方法
Abstract:
采用吸附固定化细胞技术发酵生产捷安肽素.首先对7种不同的吸附性固体载体进行筛选，发现木屑作为载体效果最好；然后通过正交优化实验和单因素实验确定载体木屑的最佳条件为：粒度0.5~0.6mm，添加浓度10gl-1发酵液.为避免吸附法固定的菌体细胞从载体上脱落，尝试在木屑固定化细胞体系中加入粘稠剂以增强吸附性能，运用单因素实验确定了粘稠剂的种类和浓度，即为15gl-1海藻酸钠.最后应用响应面方法优化固定化细胞发酵培养基及培养条件，结果表明：接种量为10%、黄豆粉水解液总氮浓度为1.7gl-1发酵液、葡萄糖浓度为33.7gl-1发酵液时捷安肽素的生物效价最大，达到7282.08u，比非固定化细胞培养提高了53.9%.图3表7参17
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