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OALib Journal期刊
ISSN: 2333-9721
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应用平板显色法测定单宁酶活力
DOI: 10.3724/SP.J.1145.2010.00883, PP. 883-887
Keywords: 单宁酶,溴甲酚绿,单宁酸,琼脂,平板显色法
Abstract:
为直观、批量、准确测定单宁酶的活力，以单宁酸为底物，溴甲酚绿为显色剂，采用平板显色法测定单宁酶的活力.对单宁酸浓度、溴甲酚绿浓度、琼脂浓度、酶加量以及反应时间的分析结果显示，平板组成为1%单宁酸、0.001%溴甲酚绿、3%琼脂，酶加量为30μl，40℃下反应24h，透明圈清晰度最高，获得标准曲线的决定系数为98%，验证反应结果显示该方法准确、稳定.图7表1参11
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