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OALib Journal期刊
ISSN: 2333-9721
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产蛋白酶北极海洋耐冷菌arcb82306的筛选及其蛋白酶性质
DOI: 10.3724/SP.J.1145.2010.00858, PP. 858-862
Keywords: 假交替单胞菌属,蛋白酶产生菌,耐冷菌,丝氨酸蛋白酶,北极
Abstract:
从北极加拿大海盆海水中分离到一株产蛋白酶耐冷菌株arcb82306.16srdna基因序列的同源性分析表明，该菌株属于假交替单胞菌属（pseudoalteromonas）.蛋白酶酶谱显示该菌株分泌多种胞外蛋白酶.胞外蛋白酶的最适作用温度和最适ph分别为45℃和9.菌株arcb82306所产蛋白酶受丝氨酸蛋白酶抑制剂pmsf抑制，金属蛋白酶抑制剂edta以及半胱氨酸蛋白酶抑制剂e-64对蛋白酶活性无影响，结果提示该蛋白酶属于丝氨酸蛋白酶.金属离子ni2+、zn2+、hg2+、cu2+、fe3+、ca2+和mg2+可部分抑制酶的活性，而dtt和巯基乙醇等还原剂及tritonx-100和吐温-80等表面活性剂对酶活性无显著影响.图6表1参16
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