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OALib Journal期刊
ISSN: 2333-9721
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产琥珀酸重组大肠杆菌的构建和发酵性能
DOI: 10.3724/SP.J.1145.2010.00851, PP. 851-857
Keywords: 琥珀酸,重组大肠杆菌,代谢调控,基因敲除,xer/dif重组酶系统
Abstract:
琥珀酸是一种重要的化工产品，微生物发酵法生产琥珀酸具有广阔的应用前景.野生大肠杆菌厌氧发酵产物中琥珀酸含量低，且有大量副产物.为构建高产琥珀酸的重组大肠杆菌，阻断代谢旁路，利用xer/dif重组酶系统对escherichiacolicicimb0013进行代谢途径的调控研究.结果显示，敲除了包括乙酸激酶和磷酸乙酰转移酶基因（acka-pta）、乳酸脱氢酶基因（ldha）、丙酮酸甲酸裂解酶基因（pflb）、乙醇脱氢酶基因（adhe）等琥珀酸合成竞争途径中关键酶基因的重组大肠杆菌菌株e.colicicimb0013-1040，其发酵液中琥珀酸成为主要产物得到积累；进一步对其pts系统进行修饰并适当增强pep羧化酶基因（ppc）表达剂量，成功获得一株具备较强琥珀酸生产能力的菌株e.colicicimb0013-1050（pth-ppc），该菌株厌氧转化葡萄糖36h，琥珀酸产量达到36.2g/l，生产强度为1.01gl-1h-1，葡萄糖-琥珀酸转化率为64.3%，发酵液经高效液相色谱（hplc）检测，无乳酸、乙酸、甲酸、乙醇生成.图7表4参18
| [1] | 1lingt(凌关庭).handbookoffoodadditives.2nded.beijing:chemicalindustrypress(北京:化学工业出版社),1991.638~639
|
| [2] | 2zeikusjg,jainmk,elankovanp.biotechnologyofsuccinicacidproductionandmarketforderivedindustrialproducts.applmicrobiolbiotechnol,1999,51(5):545~525
|
| [3] | 5sul(苏溧),chenkq(陈可泉),cait(蔡婷),wangqn(王倩楠),jiangm(姜岷).researchonanarobicculturalconditionofsuccinicacidproducingstrainactinobacillussuccinogenes130z.chinjbioprocesseng(生物加工过程),2007,5(2):67~72
|
| [4] | 8christophec,naruemolnr,joachimws.dynamicmodelingofthecentralcarbonmetabolismofescherichiacoli.biotechnolbioeng,2002,79(1):53~73
|
| [5] | 9jantamak,hauptmj,svoronossa,zhangxl,moorejc,shanmugamkt,ingramlo.combiningmetabolicengineeringandmetabolicevolutiontodevelopnonrecombinantstrainsofescherichiacolicthatproducesuccinateandmalate.biotechnolbioeng,2008,99(5):1140~1153
|
| [6] | 10jantamak,zhangxl,moorejc,shanmugamkt,svoronossa,ingramlo.eliminatingsideproductsandincreasingsuccinateyieldsinengineeredstrainsofescherichiacolic.biotechnolbioeng,2008,101(5):881~893
|
| [7] | 13sambrookj,russelldw.molecularcloning:alaboratorymanual.3rded.newyork,usa:coldspringhaborlaboratorypress,2001
|
| [8] | 14chenr,yapwmg,postmapw,baileyje.comparativestudiesofescherichiacolistrainsusingdifferentglucoseuptakesystems:metabololismsandenegretics.biotechnolbioeng,1997,56(5):583~590
|
| [9] | 3songh,leesy.productionofsuccinicacidbybacterialfermentation.enzymemicrobtechnol,2006,39:352~361
|
| [10] | 4zhull(朱蕾蕾),liuyp(刘宇鹏),zhengp(郑璞),sunzh(孙志浩).sereeningandidentincationofastrainofactinobacillussuccinogenesproducingsuccinicacidbyanaerobicfermentatfon.microbiolchina(微生物学通报),2007,34(1):80~84
|
| [11] | 6jiangm(姜岷),majf(马江锋),chenkq(陈可泉),wangyn(王益娜),yul(于丽).theprogressofrecombinantescherichiacoliforproductionofsuccinicacid.microbiolchina(微生物学通报),2009,36(1):120~124
|
| [12] | 7linh,bennettgn,sanky.effectofcarbonsourcesdifferinginoxidationstateandtransportrouteonsuccinateproductioninmetabolicallyengineeredescherichiacoli.jindmicrobiolbiotechnol,2005,32(3):87~93
|
| [13] | 11zhangxl,jantamak,moorejc,jarboealr,shanmugamkt,ingramlo.metabolicevolutionofenergy-conservingpathwaysforsuccinateproductioninescherichiacoli.procnatlacadsciusa,2009,106(48):20180~20185
|
| [14] | 12bloorae,cranenburghrm.anef?cientmethodofselectablemarkergeneexcisionbyxerrecombinationforgenereplacementinbacterialchromosomes.applenvironmicrobiol,2006,72(4):2520~2525
|
| [15] | 15sanchezam,bennettgn,sanky.novelpathwayengineeringdesignoftheanaerobiccentralmetabolicpathwayinescherichiacolitoincreasesuccinateyieldandproductivity.metabeng,2005,7(3):229~239
|
| [16] | 16zhangyx(张玉秀),wangj(王姣),wangd(王丹),qif(齐峰).advancementingeneticengineeringforproductionofsuccinicacidbyescherichiacoli.chinabiotechnol(中国生物工程杂志),2009,29(7):108~117
|
| [17] | 17linh,bennettgn,sanky.chemostatculturecharacterizationofescherichiacolimutantstrainsmetabolicallyengineeredforaerobicsuccinateproduction:astudyofthemodifiedmetabolicnetworkbasedonmetaboliteprofile,enzymeactivity,andgeneexpressionprofile.metabeng,2005,7:337~352
|
| [18] | 18lir,chenq,wangpg,qiqs.anovel-designedescherichiacolifortheproductionofvariouspolyhydroxyalkanoatesfrominexpensivesubstratemixture.applmicrobiolbiotechnol,2007,75(5):1103~1109
|
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