|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
均匀设计法优化樟芝产三萜液体发酵条件
DOI: 10.3724/SP.J.1145.2011.00901, PP. 901-906
Keywords: 樟芝,粗三萜,生物量,均匀设计,液体发酵,培养条件优化
Abstract:
为在兼顾生物量的基础上提高液体发酵中三萜的百分含量，首先通过单因子试验筛选出最佳的碳源、氮源、无机盐，然后采用均匀设计（ud）对其培养基配比和培养条件进行优化试验，建立适当的数学模型，并对模型进行验证确定优化条件.单因子试验结果表明玉米淀粉既能促进菌丝体的生长，又能促进三萜的合成；麸皮虽然对菌体生长略有不利，但是对三萜的积累作用特别显著；硫酸镁对菌丝体生长效果不显著，但是对三萜的合成比较有利.均匀设计试验结果表明，菌丝体干重最大的培养条件和三萜的最优培养条件相差甚远.菌丝体干重的最优培养条件为：1l培养液中玉米淀粉47g，麸皮47g，硫酸镁0.5g，初始ph3.0，培养7d；三萜最佳培养基配方为：1l培养液中玉米淀粉20g，麸皮20g，硫酸镁1.85g，初始ph3.0，培养16d.菌丝体干重在最优条件下能达到15.58(±0.37)gl-1；三萜百分含量在最优培养条件下高达6.04(±0.03)%，三萜的百分含量比基础发酵结果和报道的最高发酵结果3.18%提高了90%，因此，均匀设计法能有效优化液体发酵培养条件.图5表3参34
| [1] | 9wudp,chiangch.constituentsofantrodiacinnamomea.chinjchemsoc,1995,42:797~800
|
| [2] | 10cherngih,wudp,chianghc.triterpenoidsfromantrodiacinnamomea.phytochem,1996,41(1):263~267
|
| [3] | 11yangsw,shenyc,chench.steroidsandtriterpenoidsofantodiacinnamomea-afungusparasiticoncinnamomummicranthum.phytochem,1996,41(5):1389~1392
|
| [4] | 13shencc,kuoyc,huangrl,linlc,donmj,changtt,choucj.newergostaneandlanostanefromantrodiacamphorata.chinjmed,2003,14(4):247~258
|
| [5] | 16chenyj,choucj,changtt.methodforinhibitingtumorgrowthwithdehydrosulphurenicacidextractsfromantrodiacinnamomea.usa,20090318400,dec.24,2009
|
| [6] | 24liudz,liangyc,linsy,linys,wuwc,wench,such.antihypertensiveactivitiesofsolid-statecultureoftaiwanofunguscamphoratus(chang-chih)inspontaneouslyhypertensiverats.bioscibiotechnolbiochem,2007,71(1):23~30
|
| [7] | 26fangcj(方传记),luzx(陆兆新),sunlj(孙力军),biexm(别小妹),lüfx(吕凤霞),huangxq(黄现青).optimizationoffermentationtechnologyforlipopeptidesproducingbacteriabacillusamyloliquefacienses~2~4.chinjsciagricsin(中国农业科学),2008,41(2):533~539
|
| [8] | 27wangj,chenbl,rao.xz,huangj,lim.optimizationofculturingconditionsofporphyridiumcruentumusinguniformdesign.worldjmicrobiolbiotechnol,2007,23:1345~1350
|
| [9] | 29范青生.保健食品注册申报实用指南北京:中国轻工业出版社,2006.270
|
| [10] | 30changcy,leecl,pantm.statisticaloptimizationofmediumcomponentsfortheproductionofantrodiacinnamomeaac0623insubmergedcultures.applmicrobiolbiotechnol,2006,72:654~661.doi:10.1007/s00253-006-0325-6
|
| [11] | 1chiuhh.phylogeneticanalysisofantrodiaspeciesandantrodiacamphoratainferredfrominternaltranscribedspacerregion.antonvanleeuwen,2007,91:267~276
|
| [12] | 2changtt,chouww.antrodiacinnamomeasp.nov.oncinnamomumkanehiraiintaiwan.mycolres,1995,99(6):756~758
|
| [13] | 3��初,��洲.�芝之王:台�樟芝.台北:元��出版社,2001.65
|
| [14] | 4徐茂�.台�森林中的��石――樟芝.台北国际食品展专刊,2001:79~85
|
| [15] | 5��初.�芝之王2:台�樟芝.台北:元��出版社,2008.87
|
| [16] | 6geethangilim,tzengym.reviewofpharmacologicaleffectsofantrodiacamphorataanditsbioactivecompounds.ecam,2009:1~15.doi:10.1093/ecam/nep108
|
| [17] | 7cherngih,chianghc,chengmc,wangy.threenewtriterpenoidsfromantrodiacinnamomea.jnatprod,1995,58:365~371
|
| [18] | 8chench,yangsw,shenyc.newsteroidacidsfromantrodiacinnamomea,afungalparasiteofcinnamomummicranthum.jnatprod1995,58:1655~1661
|
| [19] | 12shenyc,yangsw,lincs,chench,kuoyh,chencf.zhankuicacidf:newmetabolitefromformosanfungusantrodiacinnamomea.plantamed,1997,63:86~88
|
| [20] | 14gaohw(高�薇).studiesoftriterpenescontentofnewlingzhi,antrodiacamphorate,intaiwan:[masterdegreedissertation].taipei,china:medicalinstituteofnaturalchemicals,tapeimedicaluniversity,1992
|
| [21] | 15yehct,raoyk,yaocj,yehcf,lich,chuangse,luongjohnht,laigm,tzengym.cytotoxictriterpenesfromantrodiacamphorataandtheirmodeofactioninht-29humancoloncancercells.cancerlett,2009,285(1)73-79.doi:10.1016/j.canlet.2009.05.002(advanceaccesspublishedonlineonmay23,2009)
|
| [22] | 17chenyj,choucj,changtt.compoundmmh01possessestoxicityagainsthumanleukemiaandpancreaticcancercells.toxicolvitro,2009,23:418~422
|
| [23] | 18hsiehyc,raoyk,wucc,huangcyf,geethangilim,hsusl,tzengym.methylantcinateafromantrodiacamphorateinducesapoptosisinhumanlivercancercellsthroughoxidant-mediatedcofilin-andbax-triggeredmitochondrialpathway.jchemrestoxicol,2010,23:1256~1267
|
| [24] | 19chenjj,linwj,liaoch,shiehpc.anti-inflammatorybenzenoidsfromantrodiacamphorata.jnatprod,2007,70:989~992
|
| [25] | 20shenyc,wangyh,chouyc,chencf,linlc,changtt.evaluationoftheanti-inflammatoryactivityofzhankuicacidsisolatedfromthefruitingbodiesofantrodiacamphorata.plantamed,2004,70:310~314
|
| [26] | 21shenyc,chencf,wangyh,changtt,choucj.evaluationoftheimmuno~modulatingactivityofsomeactiveprinciplesisolatedfromthefruitingbodiesofantrodiacamphorata.chinjpharmaceu,2003,55(5):313~318
|
| [27] | 22yuyl,chenih,shenky,huangry,wangwr,choucj,changtt,chucl.atriterpenoidmethylantcinatekisolatedfromantrodiacinnamomeapromotesdendriticcellactivationandth2differentiationeurjimmunol,2009,39(9):2482~2491
|
| [28] | 23songty,yengc.protectiveeffectsoffermentedfiltratefromantrodiacamphoratainsubmergedcultureagainstccl4-inducedhepatictoxicityinrats.jagricfoodchem,2003,51(6):1571~1577
|
| [29] | 25taosa(陶申傲),caiyf(蔡永峰),yuegh(岳国海),chengc(程池).studyonliquidfermentationmediumofgloeostereumincarnatumitoetimaibyuniformdesign.chinjfood&fermentind,2008,34(1):73~76
|
| [30] | 28xucp,yunjw.optimizationofsubmerged-cultureconditionsformyceliagrowthandexo-biopolymerproductionbyauriculariapolytricha(woodearsfungus)usingthemethodsofuniformdesignandregressionanalysis.biotechnolapplbiochem,2003,38:193~199
|
| [31] | 31yangfc,huanghc,yangmj.theinfluenceofenvironmentalconditionsonthemycelialgrowthofantrodiacinnamomeainsubmergedcultures.enzymeµbttechnol,2003,33:395~402
|
| [32] | 32songar(宋爱荣).thestudyonutilizationofnsourcesbyantrodiacomphora.jfungalres,2004,2(1):45~48
|
| [33] | 33shihil,pank,hsiency.influenceofnutritioncomponentsandoxygensupplyonthemycelialgrowthandbioactivemetabolitesproductioninsubmergedcultureofantrodiacinnamomea.procbiochem,2006,41:1129~1135
|
| [34] | 34lines,sungsc.cultivatingconditionsinfluenceexopolysaccharideproductionbytheediblebasidiomyceteantrodiacinnamomeainsubmergedculture.internjfoodmicrobiol,2006,108:182~187
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
