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OALib Journal期刊
ISSN: 2333-9721
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利用响应面优化klebsiellavariicola对麻疯树籽饼粕佛波酯的固态发酵降解
DOI: 10.3724/SP.J.1145.2014.12036, PP. 431-438
Keywords: 麻疯树籽饼粕,佛波酯,klebsiellavariicola,固态发酵,curcin
Abstract:
为降解麻疯树籽饼粕中的毒性成分佛波酯，利用klebsiellavariicola进行固态发酵.采用单因素实验法研究了发酵温度、接种量、初始加水量、发酵时间对佛波酯降解率的影响.在此实验结果基础上，利用designexpert8.0软件进行分析建立了发酵温度、接种量、初始加水量3个因素对佛波酯降解率影响的优化模型.该模型极显著（p<0.001），拟合度良好.经实验证实，在发酵温度为37℃、接种量33%（v/m）、初始加水量为105%（v/m）条件下发酵48h，佛波酯降解率可达到84.30%，另一种毒性物质curcin的降解率可达63.21%.研究表明，利用响应面优化k.variicola固态发酵麻疯树籽饼粕过程，既可提高佛波酯降解率，也可降解curcin，具有生产指导意义.
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