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OALib Journal期刊
ISSN: 2333-9721
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一种田头菇属真菌新记录种的生物学与产酶特性
DOI: 10.3724/SP.J.1145.2014.00148, PP. 148-151
Keywords: 菌核田头菇,生物学特性,产酶特性,漆酶,植酸酶,核糖核酸酶
Abstract:
田头菇是重要的经济食用菌类型，目前我国报道种仅有10个.为驯化栽培和开发利用新的田头菇属食用菌种质资源，以采集自北京延庆的我国新记录种菌核田头菇（agrocybetuberosa）gsm-12菌株为材料，研究了菌丝生长的最适碳源、氮源、c/n比、最适温度、最适ph值等生物学特性，以及菌丝体液体发酵产漆酶、蛋白酶、植酸酶和核糖核酸酶的特性.结果显示，菌丝生长的最适碳源为蔗糖，最适氮源为黄豆粉，最适c/n比为20/1-30/1，最适温度为24℃，最适ph为7.0.产酶特性研究显示，gsm-12菌株具有漆酶、植酸酶和核糖核酸酶活性，菌丝体中酶活较高，分别为（77.28±10.44）u/mg、（61.01±2.71）u/mg和（21.13±0.60）u/mg.本研究表明菌株gsm-12可以利用常规营养元素实现营养生长，同时菌丝体液体发酵具有良好的产酶特性.
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