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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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鼠tcp11l1基因的结构与表达分析
DOI: 10.3724/SP.J.1145.2008.00783, PP. 783-786
Keywords: tcp11l1基因,亚细胞定位,表达分析,跨膜分析
Abstract:
为了分析鼠tcp11l1基因的结构和表达，从小鼠睾丸组织总cdna中扩增鼠tcp11l1基因的开读框架(openreadingframe，orf)，定向克隆到真核表达载体pegfp-n1，构建融合表达质粒ptcp11l1-egfp，转染hek293细胞，荧光显微镜下观察tcp11l1基因的亚细胞定位；设计跨小鼠tcp11l1基因两个外显子的引物，rt-pcr分析此基因在小鼠各组织中的表达情况.并利用生物信息学的方法对鼠tcp11l1基因的结构进行初步预测.转染ptcp11l1-egfp后在胞质中能明显看到绿色荧光信号，而在胞核和胞膜中无绿色荧光信号，表明鼠tcp11l1蛋白定位于细胞质；rt-pcr分析结果表明，鼠tcp11l1基因在小鼠各组织中广泛表达.生物信息学结果表明，鼠tcp11l1和鼠tcp11的蛋白具有相同的tcp11结构域，不能确定是否存在跨膜序列.鼠tcp11l1基因和鼠tcp11基因具有相似的亚细胞定位和tcp11结构域，表明这两个基因可能具有相似的受体功能.但是与tcp11特异表达于睾丸组织的延长精细胞和精子不同，tcp11l1为广泛表达，说明tcp11l1可能在多种组织细胞中发挥作用.图6参10
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