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OALib Journal期刊
ISSN: 2333-9721
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温度两阶段控制策略发酵生产重组角质酶
DOI: 10.3724/SP.J.1145.2009.00730, PP. 730-733
Keywords: 角质酶,重组枯草芽孢杆菌,温度两阶段控制,比生长速率,比合成速率
Abstract:
为进一步提高重组bacillussubtiliswshb06-07发酵生产角质酶的产量和生产强度，在ph两阶段控制策略的基础上，考察了温度(27~40℃)对菌体生长和产酶的影响.研究发现，37℃适于菌体生长而30℃适于菌体产酶.通过分析发酵过程中菌体比生长速率及产物比合成速率的变化，确定了温度两阶段控制策略，即0~4h时控制温度37℃，4h后将温度调至30℃.通过采用这一优化策略，角质酶酶活和生产强度分别可达312.5u/ml和13.02kul-1h-1，相比恒定温度37℃控制模式下分别提高了83.4%和10.9%.图6表2参13
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