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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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甘薯lea2基因的克隆与表达分析
DOI: 10.3724/SP.J.1145.2014.00204, PP. 204-210
Keywords: 甘薯,lea2,基因克隆,数字表达谱,盐胁迫
Abstract:
胚胎发育晚期丰富蛋白（lateembryogenesisabundant,lea）是植物在逆境胁迫下产生的一种应激蛋白质，具有较高的亲水性和热稳定性，与植物抗逆功能密切相关，研究lea家族基因对于甘薯抗逆性分子育种具有重要意义.通过对甘薯（徐薯18）转录组数据库搜寻时发现编码lea2的转录本，随后对该转录本进行了克隆和测序分析，利用数字表达谱（digitalgeneexpressionprofiling，dge）和半定量rt-pcr分析了ib-lea2在不同组织和不同发育阶段的表达图谱.为进一步了解ib-lea2在生物体响应盐胁迫中的作用，将ib-lea2连接原核表达载体pet-32a，用大肠杆菌bl21（de3）菌株进行盐胁迫的耐受分析.结果显示：ib-lea2完整的开放阅读框（orf）长度为942bp，编码313个氨基酸残基.进一步研究发现，在甘薯组织内同时存在3个高度同源的lea2基因型(核苷酸序列＞99%)，分别命名为ib-lea2a、ib-lea2b和ib-lea2c.数字表达谱和半定量rt-pcr的结果显示ib-lea2在不同组织和发育阶段具有不同的表达图谱，在茎中的表达量远远高于叶和根.大肠杆菌bl21（de3）菌株盐胁迫的结果显示，较之对照菌株，表达ib-lea2的菌株能够更好地耐受高浓度的nacl.研究表明，ib-lea2对盐胁迫有一定的响应能力，能够保护细胞免受高盐的毒害.
| [1] | 7fironn,labonted,villordona,mcgregorc,k?ry,pressmane.botanyandphysiology:storagerootformationanddevelopment[m].thesweetpotato,2009.13-26
|
| [2] | 12kimyh,jeongjc,parks,leehs,kwakss.molecularcharacterizationoftwoethyleneresponsefactorgenesinsweetpotatothatrespondtostressandactivatetheexpressionofdefensegenesintobaccoleaves[j].jplantphysiol,2012,169:1112-1120
|
| [3] | 13weisingk,atkinsonrg,gardnerrc.genomicfingerprintingbymicrosatellite-primedpcr:acriticalevaluation[j].genomeres,1995,4:249-255
|
| [4] | 14taox,guyh,wanghy,zhengw,lix,zhaocw,zhangyz.digitalgeneexpressionanalysisbasedonintegrateddenovotranscriptomeassemblyofsweetpotato[ipomoeabatatas(l.)lam.][j].plosone,2012,7:e36234
|
| [5] | 15robinsonmd,mccarthydj,smythgk.edger:abioconductorpackagefordifferentialexpressionanalysisofdigitalgeneexpressiondata[j].bioinformatics,2010,26:139-140
|
| [6] | 16liy,guyh,qinh,zhangyz.twopairsofsucrosetransportersinipomoeabatatas(l.)lamarepredominantlyexpressedinsinkleavesandsourceleavesrespectively[j].plantsci,2010,179:250-256
|
| [7] | 20krishnanr,magoonml,kvb.cytologicalevidenceontheoriginofsweetpotato[j].theorapplgenet,1970,40:360-366
|
| [8] | 21guanzy,suyj,tengnj,chensm,sunhn,licl,chenfd.morphological,physiological,andstructuralresponsesoftwospeciesofartemisiatonaclstress[j].sciworldj,2013,2013:309808
|
| [9] | 22sahic,singha,kumark,blumwalde,grovera.salttoleranceresponseinrice:genetics,molecularbiology,andcomparativegenomics[j].functintegrgenomics,2006,6:263-284
|
| [10] | 23lany,caid,zhengyy,expressioninescherichiacoliofthreedifferentsoybeanlateembryogenesisabundant(lea)genestoinvestigateenhancedstresstolerance[j].jintegplantbiol,2005,47(5):613-621
|
| [11] | 25palakolanusr,gudamr,prachip,kottakotac,malireddykr.cloningandmolecularcharacterizationofageneencodinglateembryogenesisabundantproteinfrompennisetumglaucum:protectionagainstabioticstresses[j].molbiolrep,2012,39:7163-7174
|
| [12] | 1刘洋,邢鑫,李德全.lea蛋白的分类与功能研究进展[j].生物技术通报,2011,8:36-43[liuy,xingx,lidq.studiesontheclassificationandfunctionofleaproteins[j].biotechlbull,2011,8:36-
|
| [13] | 2closetj.dehydrins:emergenceofabiochemicalroleofafamilyofplantdehydrinsproteins[j].physiolplant,1996,97(4):795-803
|
| [14] | 3jayaprakashtl,ramamohang,krishnaprasadbt,ganeshkumar,prasadtg,mathewmk,udayakumarm.genotypicvariabilityindifferentialexpressionoflea2andlea3genesandproteinsinresponsetosalinitystressinfingermillet(eleusinecoracanagaertn)andrice(oryzasatival.)seedlings[j].annbot,1998,82:513-522
|
| [15] | 4soulagesjl,kimk,arreseel,waltersc,cushmanjc.conformationofagroup2lateembryogenesisabundantproteinfromsoybean.evidenceofpoly(l-proline)-typeiistructure[j].plantphysiol,2003,131:963-975
|
| [16] | 5kasugam,miuras,shinozakik,yamaguchi-shinozakik.acombinationofthearabidopsisdreb1ageneandstress-induciblerd29apromoterimproveddrought-andlow-temperaturestresstoleranceintobaccobygenetransfer[j].plantcellphysiol,2004,3(45):346-350
|
| [17] | 6camposf,zamudiof,covarrubiasaa.twodifferentlateembryogenesisabundantproteinsfromarabidopsisthalianacontainspecificdomainsthatinhibitescherichiacoligrowth[j].biochembiophysrescommun,2006,342:406-413
|
| [18] | 10parksc,kimyh,jeongjc,kimcm,leehs,bangjw,kwakss.sweetpotatolateembryogenesisabundant14(iblea14)geneinfluenceslignificationandincreasesosmotic-andsaltstress-toleranceoftransgeniccalli[j].planta,2011,233:621-634
|
| [19] | 11senthilkumarr,yehkw.multiplebiologicalfunctionsofsporaminrelatedtostresstoleranceinsweetpotato(ipomoeabatataslam)[j].biotechnoladv,2012,30:1309-1317
|
| [20] | 17changmj,taox,guyh,lingx,shaohh,caoqh,zhangyz.cloningandcharacterizationofthe14-3-3proteingenefromipomoeabatatas(l.)lam[j].afrjmicrobiolres,2012,6(9):1990-1999
|
| [21] | 18caoqh,shaohh,guyh,taox,huangcl,zhangyz.cloning,characterizationandexpressionanalysisofhsp70genefromsweetpotato[j].afrjmicrobiolres,2012,6(34):6325-6332
|
| [22] | 19jiangys,wangjx,taox,zhangyz.characterizationandexpressionofrubiscoactivasegenesofipomoeabatatas[j].molbiolrep,2013,40(11):6309-6321
|
| [23] | 24choureyk,ramanis,aptesk.accumulationofleaproteinsinsalt(nacl)stressedyoungseedlingsofrice(oryzasatival.)cultivarburarataandtheirdegradationduringrecoveryfromsalinitystress[j].jplantphysiol,2003,160:1165-1174
|
| [24] | 8padmajag.usesandnutritionaldataofsweetpotato[m].thesweetpotato,2009.189-234
|
| [25] | 9李强,刘庆昌,马代夫,臧宁.甘薯遗传转化研究现状、问题及展望[j].分子植物育种,2005,3(1):99-106[liq,liuqc,madf,zangn,houfy.advances,problemsandprospectsingenetictransformationonsweetpotato[j].molplantbreed,2005,3(1):99-
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