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OALib Journal期刊
ISSN: 2333-9721
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果糖对莆田黑猪精液中nagase的抑制动力学
DOI: 10.3724/SP.J.1145.2014.07013, PP. 46-50
Keywords: 果糖,莆田黑猪,精液,n-乙酰-β-d-氨基葡萄糖苷酶,抑制动力学
Abstract:
为了解精液中不同类型糖类物质对精子细胞中n-乙酰-β-d-氨基葡萄糖苷酶（nagase）的影响，以莆田黑猪精液nagase为材料，研究精液和精液稀释液主要成分之一果糖对酶的抑制作用.结果显示，果糖对nagase具有浓度效应（ic50=575mmoll-1）.果糖对nagase的抑制类型是可逆的非竞争性抑制，抑制常数是1.27×10-2mmoll-1.应用底物反应的动力学方法研究果糖对该酶的抑制动力学，建立了抑制动力学模型，证明了果糖对该酶的影响是快速结合再缓慢失活的过程.本研究表明，在高浓度的抑制剂溶液中nagase将完全失活，底物对其无保护作用.
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