|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
甘蔗scot-pcr反应体系优化与多态性引物筛选及应用
DOI: 10.3724/SP.J.1145.2012.00810, PP. 810-818
Keywords: 甘蔗,scot标记,体系优化,引物筛选,多态性能力
Abstract:
目标起始密码子多态性（startcondontargetedpolymorphism，scot）分子标记是一种新型的标记，结合了issr标记和rapd标记的优点.本研究针对scot-pcr反应体系的影响因素，以甘蔗叶片dna为材料，在单因子实验的基础上，采用l16(45)正交实验设计，进一步探讨了模板dna、mg2+、dntps、引物及taq酶等5个因素对甘蔗scot-pcr扩增效果的影响，建立了甘蔗scot-pcr的优化反应体系.25μlpcr反应混合液中，含50ngdna模板、2.0μl10×extaqbuffer（mg2+plus）、0.625uextaq酶，dntp和引物的终浓度分别为0.22mmol/l和0.9μmol/l.以我国种植面积最大的栽培品种新台糖22号为模板，应用优化体系，对40条scot标记引物进行测试，筛选出16条有效扩增的引物，且均为多态性引物，其gc含量在50%~67%之间.该体系的稳定性和scot标记引物的扩增能力，通过基于随机选择的4条引物对9份具有地理来源和遗传背景不同的甘蔗种质进行标记分析来验证，结果共扩增出84条带，其中多态性条带占82.14%，平均单条引物可扩增出21条.研究结果为在甘蔗上进一步开发和应用功能性scot标记奠定了基础.图9表5参23
| [1] | 2liaojq(廖江雄).researchandprospectinmolecularmarkerofcomplexpolyploidplantsofsugarcane.sugarcane(甘蔗),1998,5(2):10~15
|
| [2] | 3collardbcy,mackildj.start-codontargeted(scot)polymorphism:asimple,noveldnamarkertechniqueforgeneratinggene-targetedmarkersinplants.plantmolbiolrep,2009,27:86~93
|
| [3] | 4xiongfq(熊发前),jiangq(蒋菁),zhongrc(钟瑞春),hanzq(韩柱强),helq(贺梁琼),liz(李忠),zhuangwj(庄伟建).tangrh(唐荣华).applicationofscotmolecularmarkeringenusarachis.actaagronsin(作物学报),2010,36(12):2055~2061
|
| [4] | 5chenh(陈虎),hexh(何新华),luoc(罗聪),zhujh(朱建华),lif(李峰).analysisonthegeneticdiversityof24longan(dimocarpuslongan)accessionsbyscotmarkers.actahorticsin(园艺学报),2010,37(10):1651~1654
|
| [5] | 6xiongfq(熊发前),tangrh(唐荣华),chenzl(陈忠良),panlh(潘玲华),zhuangwj(庄伟建).scot:anovelgenetargetedmarkertechniquebasedonthetranslationstartcodon.molplantbreed(分子植物育种),2009,7(3):635~638
|
| [6] | 7carrollb,mclntyrel,berdingn.finalreport-anassessmentoftheapplicationofdnamarkerstostudiesofgeneticdiversityandmarkerassistedselectioninsugarcane.bsespublication,1999.1~12
|
| [7] | 9lim(李鸣),liangcx(梁朝旭),fangwk(方位宽),liangj(梁俊),liyr(李杨瑞,),tanym(谭裕模),hewz(何为中).aflpmolecularmarkertechnologyanditsapplicationinsugarcanegeneticsandbreeding.sugarcropschina(中国糖料),2006(1):43~46
|
| [8] | 10nairnv,selvia,sreenivasantv,pushpalathakn.moleculardiversityinindiansugarcanecultivarsasrevealedbyrandomlyamplifieddnapolymorphisms.euphytica,2002,127(2):219~225
|
| [9] | 15kwoks,kelloggde,mckinneyn,spasicd,godad,levensonc.effectsofprimer-templatemismatchesonthepolymerasechainreaction:humanimmunodeficiencyvirustype1modelstudies.nucleicacidres,1990,18(4):999~1005
|
| [10] | 16jiangf(蒋锋),liupf(刘鹏飞),dusz(杜世州),wangxm(王晓明).researchprogressoncropqtlpappingmethod.janhuiagricsci(安徽农业科学),2009,37(26):12496~12497,12508
|
| [11] | 17mingdx(明道绪).thefieldexperimentandstatisticsanalysis.beijing:sciencepress(北京:科学出版社),2005.294
|
| [12] | 18wangsq(王水琦),linyx(林一心),yexl(叶小丽).molecularmarkersanditsapplicationinsugarcanebreeding.sugarcanecanesugar(甘蔗糖业),2003(4):21~25
|
| [13] | 19chenh(陈辉),fanyh(范源洪),caiq(蔡青).rapdmolecularmarkertechnologyanditsapplicationinsugarcanebreeding.sugarcropschina(中国糖料),2004(1):46~48
|
| [14] | 20zhuyf(朱岩芳),zhusj(祝水金),liyp(李永平),mawg(马文广),zhengjy(郑钧晔),huj(胡晋).applicationofissrmolecularmarkerinplantgermplasmresearch.seed(种子),2010,29(2):55~59
|
| [15] | 21liuj(刘静).thedevelopmentandapplicationofaflp.shandongagricsci(山东农业科学),2010(5):10~14
|
| [16] | 22zhangjc(张君诚),songyh(宋育红),zhuy(朱勇),zhanghy(张杭颖),zhongy(钟扬).aflpanalysisofgeneticdiversityandpopulationstructureofhuperziaserrata(thunb.exmurray)trev.var.longipetiolata(spring)h.m.chang.chinjapplenvironbiol(应用与环境生物学报),2011,17(1):18~23
|
| [17] | 23chengxl(陈香玲),liyr(李杨瑞),yanglt(杨丽涛),wujm(吴建明),xiongfq(熊发前),luoc(罗聪).comparisonoftwoelectrophoresismethodsincdna-scotgenedifferentialexpression.biothchnolbull(生物技术通报),2010(10):93~95
|
| [18] | 1chenrk(陈如凯).theoryandpracticeofmodernsugarcanebreeding.beijing:chinaagriculturalpress(北京:中国农业科学院),2003.1~2
|
| [19] | 8laofy(劳方业),liur(刘睿),hehy(何慧怡),denghh(邓海华),liqw(李奇伟),chenzh(陈仲华),chenjw(陈健文),fuc(符成),qiyw(齐永文),zhangcm(张垂明).geneticdiversityanalysisofsugarcaneparentswithaflpinchina.genomics&genomapplbiol(基因组学与应用生物学),2009,28(3):503~508
|
| [20] | 11xulp(许莉萍),chenrk(陈如凯).identificationofrapdmarkerlinkedtosmutresistancegeneinsugarcane.chinjapplenvironbiol(应用与环境生物学报),2004,10(3):263~267
|
| [21] | 12cordeirogm,panyb,henryrj.sugarcanemicrosatellitesfortheassessmentofgeneticdiversityinsugarcanegermplasm.plantsci,2003,165(1):181~189
|
| [22] | 13yual(俞爱丽),zhangmq(张木清),chenrk(陈如凯).applicabilityofinter-simplesequencerepeatpolymorphismsinsugarcaneanditsrelatedgeneraasdnamarkers.jfujianagricforuniv(福建农林大学学报),2002,31(4):484~489
|
| [23] | 14queyx(阙友雄),chents(陈天生),xulp(许莉萍).geneticdiversityofimportantsugarcanegermplasmontrapmarker.jagricbiotechnol(农业生物技术学报),2009(3):496~503
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
