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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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一种野生香蘑的分离、鉴定、培养条件与产酶特性
DOI: 10.3724/SP.J.1145.2012.00804, PP. 804-809
Keywords: 紫丁香蘑,its鉴定,培养条件,漆酶,cu2+
Abstract:
为充分开发利用野生食用菌资源，从采集自北京怀柔的野生香蘑子实体中分离获得纯培养，编号为lepistasp.gsm-11，结合形态学特征与its序列分析鉴定其分类学地位，并进一步研究其菌丝生长的最适碳源、氮源、c/n比、生长因子、最适温度和最适ph值，并测定了菌体液体发酵产漆酶、蛋白酶、植酸酶、核糖核酸酶和凝集素的特性，同时还研究了cu2+对漆酶产量的影响.结果表明，该菌株与genbank中报道的采集自中国辽宁、美国马里兰州和日本宫城的紫丁香蘑（lepistanuda）同源性最高，分别为100%、99.98%和99.93%，确定该野生香蘑为紫丁香蘑（its序列genbank登录号jq519375）；菌丝体生长的最适碳源为蔗糖，最适氮源为酵母浸膏，最适c/n比为20/1，最适生长因子为维生素c，最适温度为24℃，最适ph为7.0；gsm-11菌株菌丝体和发酵液中均具有漆酶活性，但缺乏其他3种酶和凝集素活性.1.0~2.0mmol/l的cu2+对漆酶分泌有显著的促进作用，其活性达到对照组的149.2%~196.4%.图2表6参26
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